506-05A

Human Lung Fibroblasts: HLF, adult

• Synonim(y): HLF, HLF cells
• Kod UNSPSC: 41106514
• NACRES: NA.81

Właściwości

• pochodzenie biologiczne: human lung (normal parenchyma)
• Poziom jakości: 100
• opakowanie: pkg of 500,000 cells
• producent / nazwa handlowa: Cell Applications, Inc
• tryb wzrostu: Adherent
• kariotyp: 2n = 46
• morfologia: fibroblast
• metody: cell culture | mammalian: suitable
• powiązane choroby: asthma; tuberculosis; cardiovascular diseases; cancer
• Warunki transportu: dry ice
• temp. przechowywania: −196°C

Opis

Opis ogólny

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HLF provide an excellent model system to study many aspects of human pulmonary function and pathophysiology.

HLF have been utilized in a number of research publications, for example to:

• Demonstrate differential gene expression patterns between fetal and adult lung fibroblasts, in particular showing that fetal fibroblasts express higher level of TGF-β receptor interacting protein 1 (TRIP-1) and consequently are less able to contract collagen than adult lung fibroblasts, leading to better healing of acute injuries in very immature lungs compared to higher levels of fibrosis observed in older patients (Navarro, 2009), and additionally discover that TRIP-1 regulates lung fibroblast/myofibroblast trans-differentiation (Nyp, 2014)
• Show that angiotensin II promotes lung fibrosis by activating expression of IL-4, IL-7, and PDGF-D increasing the extracellular matrix formation, and that this process can be blocked by angiotensin receptor inhibitor candesartan (Okada, 2009)
• Determine that phosphorylation of C/EBPβ contributes to lung fibrosis (Buck, 2011)
• Demonstrate that increased tissue rigidity activates toll-like receptor 2 and NF-κB in lung fibroblasts and induces expression of cytokines, CXCL1-3, IL-8 and TNF-α (Zheng, 2011)
• Demonstrate that 2-ethoxyestradiol, a non-estrogenic estradiol metabolite analog, inhibits cell growth and retards the progression of pulmonary fibrosis and hypertension (Tofovic, 2008, 2009)
• Identify the hypoxia-induced ROS/RhoA–ROCK dependent actin reorganization as a mediator of myofibroblast transformation and collagen synthesis in lung fibrosis (Ni, 2013)

Pochodzenie linii komórkowej

Lungs

Zastosowanie

pulmonary function, gene expression, healing and repair process, differentiation, extracellular matrix production, growth factor and cytokine activation, actin reorganization, synthesis of type II collagen, elastin, and proteoglycans, airway remodeling, pharmaceutical development

Komponenty

Basal Medium containing 10% FBS & 10% DMSO

Uwaga dotycząca przygotowania

• 1st passage, >500,000 cells in Basal Medium containing 10% FBS & 10% DMSO
• Can be cultured at least 12 doublings

Rutyna subkultury

Please refer to the HLF Culture Protocol.

Oświadczenie o zrzeczeniu się odpowiedzialności

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 12 - Non Combustible Liquids
• Klasa zagrożenia wodnego (WGK): WGK 1
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable