T47D Cell Line human

85102201, human breast, Epithelial

• Synonim(y): T-47-D Cells, T-47D Cells, T47D:A Cells
• Kod UNSPSC: 41106514

Właściwości

• product name: T47D Cell Line human, 85102201
• pochodzenie biologiczne: human breast
• opis: Human breast tumor
• tryb wzrostu: Adherent
• kariotyp: 2n = 46, hypertriploid, modal no. 65
• morfologia: Epithelial
• produkty: Not specified
• receptory: Not specified
• metody: cell culture | mammalian: suitable
• powiązane choroby: cancer
• Warunki transportu: dry ice
• temp. przechowywania: −196°C

Opis

Pochodzenie linii komórkowej

Human breast tumour

Opis linii komórkowej

Established from the pleural effusion of a ductal carcinoma of the breast of a 54-year-old female. The cells carry receptors for a variety of steroids.

Zastosowanie

T47D cell line human has been used:

• to study the effects of a polysaccharide–protein complex (PPC) isolated from metabolites of gut bacteria Raoultella ornithinolytica from the earthworm Dendrobaena veneta against Candida albicans
• has been cultured and incubated with bone marrow-derived macrophages to study their migration and podosome formation
• to study the anti-proliferative effects of two novel classes of potential antimetabolites on T47D cells

Profil DNA

STR-PCR Data: Amelogenin: X
CSF1PO: 11,13
D13S317: 12
D16S539: 10
D5S818: 12
D7S820: 11
THO1: 6
TPOX: 11
vWA: 14

pożywka hodowlana

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Rutyna subkultury

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Inne uwagi

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