M4655

Minimum Essential Medium Eagle

With Earle′s salts, L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

• Synonim(y): EMEM, MEM
• Kod UNSPSC: 12352207
• NACRES: NA.75

Właściwości

• Poziom jakości: 400
• sterylność: sterile-filtered
• Postać: liquid
• metody: cell culture | mammalian: suitable
• zanieczyszczenia: endotoxin, tested
• komponenty: sodium pyruvate: no; phenol red: yes; Earle’s salts (5% CO₂): yes; HEPES: no; NaHCO₃: yes; L-glutamine: yes
• Warunki transportu: ambient
• temp. przechowywania: 2-8°C

Opis

Opis ogólny

Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media. Early attempts to cultivate normal mammalian fibroblasts and certain subtypes of HeLa cells revealed they had specific nutritional requirements that could not be met by Eagle′s Basal Medium (BME). Subsequent studies using these and other cells in culture indicated additions to BME could be made to aid growth of a wider variety of fastidious cells.

MEM, which incorporates these modifications, includes higher concentrations of amino acids so the medium more closely approximates the protein composition of cultured mammalian cells. MEM has been used for cultivation of a wide variety of cells grown in monolayers. Optional supplementation of non-essential amino acids to the formulations that incorporate either Hanks′ or Earle′s salts has broadened the usefulness of this medium.

Zastosowanie

Generally used to grow attached cell lines, such as fibroblasts, in the presence of FBS, calf or horse sera.

Inne uwagi

This product lacks L-Ala; L-Asn; L-Glu; Gly; L-Pro; L-Ser.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 12 - Non Combustible Liquids
• Klasa zagrożenia wodnego (WGK): WGK 1
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable