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M4526

Sigma-Aldrich

Minimum Essential Medium Eagle

Alpha Modification, with sodium bicarbonate and Earl's salts, without ʟ-glutamine, ribonucleosides and deoxyribonucleosides, liquid, sterile-filtered, suitable for cell culture

Synonim(y):

αMEM, EMEM, MEM

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About This Item

Kod UNSPSC:
12352207
NACRES:
NA.71

product name

Minimum Essential Medium Eagle, Alpha Modification, with sodium bicarbonate, without L-glutamine, ribonucleosides and deoxyribonucleosides, liquid, sterile-filtered, suitable for cell culture

Poziom jakości

400

sterylność

sterile-filtered

Postać

liquid

metody

cell culture | mammalian: suitable

zanieczyszczenia

endotoxin, tested

komponenty

Earle’s salts (5% CO2): yes
sodium pyruvate: yes
glucose: yes
L-glutamine: no
HEPES: no
Hanks’ salts (2% CO2): no
phenol red: yes
NaHCO3: yes
stable glutamine: no

Warunki transportu

ambient

temp. przechowywania

2-8°C

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Opis ogólny

Minimum Essential Medium Eagle (MEM) is a synthetic cell culture media, developed by Harry Eagle. It has higher concentrations of amino acids so the medium more closely approximates the protein composition of cultured mammalian cells. Optional supplementation of non-essential amino acids to the formulations that incorporate either Hanks′ or Earle′s salts has enhanced the uses of this medium. The α modification of MEM with Earle′s balanced salts also known as αMEM, contains non-essential amino acids, sodium pyruvate, and additional vitamins.
This is the most enriched variation of the MEM formulation offered. It contains all 21 normal amino acids, some at increased concentrations. In addition, it contains 5 additional vitamins.

Zastosowanie

Minimum Essential Medium Eagle has been used to maintain:
  • immature cumulus-oocyte complexes from mice
  • human mesenchymal stem cells (MSC)
  • mouse pre-osteoblastic cell line

Rekonstytucja

Supplement with 0.292 g/L L-glutamine.
This page may contain text that has been machine translated.

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Kod klasy składowania

12 - Non Combustible Liquids

Klasa zagrożenia wodnego (WGK)

WGK 1

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable

Certyfikaty analizy (CoA)

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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Michelle L Churchman et al.
Cancer cell, 33(5), 937-948 (2018-04-24)
Somatic genetic alterations of IKZF1, which encodes the lymphoid transcription factor IKAROS, are common in high-risk B-progenitor acute lymphoblastic leukemia (ALL) and are associated with poor prognosis. Such alterations result in the acquisition of stem cell-like features, overexpression of adhesion
Thi Thu Hien Bui et al.
Molecular reproduction and development, 84(5), 356-362 (2017-02-25)
Full-grown mouse antral oocytes are classified as surrounding nucleolus (SN) or not-surrounding nucleolus (NSN), depending on the respective presence or absence of a ring of Hoechst-positive chromatin surrounding the nucleolus. In culture, both types of oocytes resume meiosis and reach
Masaaki Kitada et al.
Glia, 67(5), 950-966 (2019-01-15)
Direct conversion is considered a promising approach to obtain tissue-specific cells for cell therapies; however, this strategy depends on exogenous gene expression that may cause undesired adverse effects such as tumorigenesis. By optimizing the Schwann cell induction system, which was
Ricardo Rodrigues-Pinto et al.
Scientific reports, 8(1), 12866-12866 (2018-08-29)
The adult nucleus pulposus originates from the embryonic notochord, but loss of notochordal cells with skeletal maturity in humans is thought to contribute to the onset of intervertebral disc degeneration. Thus, defining the phenotype of human embryonic/fetal notochordal cells is
Sarah Hemming et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 31(3), 1011-1027 (2016-12-10)
In this study, we examined the functional importance of EZH2 during skeletal development and homeostasis using the conditional deletion of
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MEM (Minimum Essential Medium Eagle)

Duży wybór preparatów MEM. Minimum Essential Medium (MEM), opracowane przez Harry'ego Eagle'a, jest jednym z najczęściej stosowanych syntetycznych podłoży do hodowli komórkowych.

Nasz zespół naukowców ma doświadczenie we wszystkich obszarach badań, w tym w naukach przyrodniczych, materiałoznawstwie, syntezie chemicznej, chromatografii, analityce i wielu innych dziedzinach.

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