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DL-Dithiothreitol solution
1 M in H2O
Synonim(y):
(±)-threo-1,4-Dimercapto-2,3-butanediol solution, DTT
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About This Item
Wzór empiryczny (zapis Hilla):
C4H10O2S2
Numer CAS:
Masa cząsteczkowa:
154.25
Beilstein:
1719757
Numer MDL:
Kod UNSPSC:
12161504
Identyfikator substancji w PubChem:
NACRES:
NA.25
Polecane produkty
Formularz
liquid
Poziom jakości
przydatność reakcji
reagent type: reductant
stężenie
1 M in H2O
gęstość
1.04 g/mL at 20 °C
1.035 g/mL at 25 °C
ciąg SMILES
O[C@H](CS)[C@H](O)CS
InChI
1S/C4H10O2S2/c5-3(1-7)4(6)2-8/h3-8H,1-2H2/t3-,4-/m1/s1
Klucz InChI
VHJLVAABSRFDPM-QWWZWVQMSA-N
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Opis ogólny
Dithiothreitol (DTT) is used in proteomics applications to maintain sulfhydryl (-SH) groups in the reduced state and for quantitative reduction of disulfide (-S-S-) groups. It is a frequently used reagent in buffers due to its ability to reduce the oxidation state of a protein sample and preserves enzymatic activity. DTT is oxidized to the cyclic disulfide during the reduction of other disulfides in the solution.
Dithiothreitol is an effective reducing agent for protein analysis. The sealed-ampule format is convenient and easy to use when preparing samples for electrophoresis or other applications and avoids waste and loss of potency.
Zastosowanie
DL-Dithiothreitol solution has been used:
- in western blot analysis
- as a supplement in lysis buffer to study its effects on sperm lysis
- as a component of cold homogenization buffer, and chilled wash buffer for the isolation of nuclei from frozen rat tissues
- in sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), chromatography, modification of cysteine-containing compounds
Cechy i korzyści
Low toxicity and reduced odor compared to other thiol compounds.
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wyposażenie dodatkowe
Hasło ostrzegawcze
Danger
Zwroty wskazujące rodzaj zagrożenia
Zwroty wskazujące środki ostrożności
Klasyfikacja zagrożeń
Eye Dam. 1 - Skin Irrit. 2
Kod klasy składowania
12 - Non Combustible Liquids
Klasa zagrożenia wodnego (WGK)
WGK 2
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Środki ochrony indywidualnej
Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter
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