DTT-RO

DTT

crystalline powder, =97% (Ellman′s reagent), M_r 154.3

• Synonim(y): DL-Dithiothreitol, 1,4-dithiothreitol, cleland′s reagent, dithiothreitol, 1,4-, threo-1,4-Dimercapto-2,3-butanediol, Cleland’s reagent, DTT
• Wzór liniowy: HSCH₂CH(OH)CH(OH)CH₂SH
• Numer CAS: 3483-12-3
• Masa cząsteczkowa: 154.25
• Beilstein: 1719757
• Numer MDL: MFCD00004877
• Kod UNSPSC: 12352200
• Identyfikator substancji w PubChem: 329798930

Właściwości

• opis: 1,4-Dithiothreitol
• Próba: 97% (Ellman′s reagent)
• Postać: crystalline powder
• masa cząsteczkowa: M_r 154.3
• przydatność reakcji: reagent type: reductant
• opakowanie: pkg of 10 g (10708984001); pkg of 2 g (10197777001); pkg of 25 g (11583786001)
• producent / nazwa handlowa: Roche
• pH: 5.1 (20 °C)
• mp: 41-44 °C (lit.)
• temp. przechowywania: 2-8°C
• ciąg SMILES: O[C@H](CS)[C@H](O)CS
• InChI: 1S/C4H10O2S2/c5-3(1-7)4(6)2-8/h3-8H,1-2H2/t3-,4-/m1/s1
• Klucz InChI: VHJLVAABSRFDPM-QWWZWVQMSA-N

Opis

Opis ogólny

1,4-dithiothreitol is commonly known as Cleland′s reagent. It confers protection to thiol groups and reduces disulfide bonds in peptides and proteins. It reduces disulfide bonds to sulfhydryl group and is commonly used in receptor studies, to determine the functional importance of disulfide bonds during receptor occupancy and functionality. It also maintains monothiols completely in the reduced state.

Zastosowanie

1,4-dithiothreitol is used in:

• Isolation, purification and characterization of proteins and enzymes
• Measurement of enzyme activities (reactivation of enzymes)
• Determination of disulfide groups in proteins and enzymes
• DNA extraction prior to amplification

An excellent reagent for maintaining SH groups in reduced state; quantitatively reduces disulfides. DTT is effective in sample buffers for reducing protein disulfide bonds prior to SDS-PAGE. DTT can also be used for reducing the disulfide bridge of the cross-linker N,N′-bis(acryloyl)cystamine to break apart the matrix of a polyacrylamide gel. DTT is less pungent and is less toxic than 2-mercaptoethanol. Typically, a seven fold lower concentration of DTT (100 mM) is needed than is used for 2-mercaptoethanol (5% v/v, 700 mM).

Charakterystyka techniczna

Oxidized form: <2.5% (absorbance at 283nm)

Sekwencja

Enantiomers
Our DTT-preparation is optically inactive, i.e. it is the D,L-DTT.

Uwaga dotycząca przygotowania

Storage conditions (working solution): A solution of DTT in Hepes buffer, pH 7.75 is stable for one week at 2 to 8 °C if the container is tightly sealed and the solution is protected from atmospheric oxygen by argon or nitrogen.

Inne uwagi

For life science research only. Not for use in diagnostic procedures.

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Informacja o środkach bezpieczeństwa

• Piktogramy: GHS05,GHS07
• Hasło ostrzegawcze: Danger
• Zwroty wskazujące rodzaj zagrożenia: H302 - H315 - H318 - H412
• Zwroty wskazujące środki ostrożności: P264 - P270 - P273 - P280 - P305 + P351 + P338 + P310 - P501
• Klasyfikacja zagrożeń: Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Skin Irrit. 2
• Kod klasy składowania: 11 - Combustible Solids
• Klasa zagrożenia wodnego (WGK): WGK 2