A3221

Iodoacetamide

Single use vial of 56 mg

• Synonim(y): 2-Iodoacetamide, IAA, Monoiodoacetamide, alpha-Iodoacetamide
• Wzór liniowy: ICH₂CONH₂
• Numer CAS: 144-48-9
• Masa cząsteczkowa: 184.96
• Beilstein: 1739080
• Numer WE: 205-630-1
• Numer MDL: MFCD00008028
• Kod UNSPSC: 12352111
• Identyfikator substancji w PubChem: 24890731
• NACRES: NA.56

Właściwości

• pochodzenie biologiczne: synthetic (organic)
• Poziom jakości: 200
• Próba: ≥99% (HPLC)
• Postać: powder
• opakowanie: vial of 56 mg (Single use)
• warunki przechowywania: (Tightly closed. Dry. Keep in a well-ventilated place. Keep locked up or in an area accessible; only to qualified or authorized persons.)
• kolor: white to off-white
• mp: 92-95 °C (lit.)
• rozpuszczalność: H₂O: soluble 50 mg/mL, clear, colorless to faintly yellow
• przydatność: suitable for LC-MS
• Zastosowanie: microbiology
• temp. przechowywania: 2-8°C
• ciąg SMILES: NC(=O)CI
• InChI: 1S/C2H4INO/c3-1-2(4)5/h1H2,(H2,4,5)
• Klucz InChI: PGLTVOMIXTUURA-UHFFFAOYSA-N

Opis

Opis ogólny

2-Iodoacetamide is a thiol-alkylating agent frequently used in peptide mapping for biochemical, cell culture and proteomics research. It functions similarly to iodoacetate, primarily binding covalently with the thiol group of cysteine in proteins, thereby preventing the formation of disulfide bonds. This attribute is crucial for several applications, including 2-D protein electrophoresis to reduce streaking and improve resolution, as an inhibitor of deubiquitinase enzymes (DUBs) and a peptidase inhibitor. It is often employed during sample preparation for de novo peptide sequencing with protein mass spectrometry.

It has specific interactions with cysteine and histidine residues in proteins, affecting enzymes like ribonuclease. While it may react slowly with histidine, its inhibitory properties contribute to the research on proteases, such as cysteine proteases. Iodoacetamide is known for its irreversibility in inhibiting enzymes and its ability to form stable protein derivatives, making it valuable in studying protein structures, protein behavior, and enzyme inhibition.

Zastosowanie

Iodoacetamide has been used:

• to prevent enzymatic disulfide reduction in the protein samples
• to inhibit glyceraldehyde-3-phosphate dehydrogenase effectively blocking ATP production in cell biology research

Działania biochem./fizjol.

Iodoacetamide acts as an alkylating reagent for cysteine residues in peptide sequencing. It is an irreversible inhibitor of enzymes with cysteine at the active site. It reacts much more slowly with histidine residues, but that activity is responsible for inhibiting ribonuclease.

Cechy i korzyści

High quality compound suitable for multiple research applications

Opakowanie

Packaged in sealed ampules

Inne uwagi

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Informacje prawne

Technology developed in partnership with Proteome Systems^™

Proteome Systems is a trademark of Proteome Systems Ltd

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Informacja o środkach bezpieczeństwa

• Piktogramy: GHS06,GHS08
• Hasło ostrzegawcze: Danger
• Zwroty wskazujące rodzaj zagrożenia: H301,H317,H334,H413
• Zwroty wskazujące środki ostrożności: P261 - P264 - P273 - P280 - P301 + P310 - P302 + P352
• Klasyfikacja zagrożeń: Acute Tox. 3 Oral - Aquatic Chronic 4 - Resp. Sens. 1 - Skin Sens. 1
• Kod klasy składowania: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• Klasa zagrożenia wodnego (WGK): WGK 3
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable
• Środki ochrony indywidualnej: Eyeshields, Faceshields, Gloves, type P2 (EN 143) respirator cartridges