MABS351-I

Anti-FAT10 Antibody, clone 4F1

• Synonim(y): Diubiquitin, UBD, Ubiquitin D
• Kod UNSPSC: 12352203
• NACRES: NA.41

Właściwości

• pochodzenie biologiczne: mouse
• Poziom jakości: 200
• forma przeciwciała: purified antibody
• rodzaj przeciwciała: primary antibodies
• klon: 4F1, monoclonal
• masa cząsteczkowa: calculated mol wt 18 kDa; observed mol wt ~18 kDa
• oczyszczone przez: using protein G
• reaktywność gatunkowa: human
• opakowanie: antibody small pack of 100
• metody: immunocytochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• izotyp: IgG2cκ
• sekwencja epitopowa: Unknown
• numer dostępu Protein ID: NP_006389
• numer dostępu UniProt: O15205
• temp. przechowywania: 2-8°C
• informacje o genach: human ... UBD(10537)

Opis

Specyficzność

Clone 4F1 is a mouse monoclonal antibody that detects FAT10/Ubiquitin D.

Immunogen

GST-tagged recombinant human FAT10/Ubiquitin D.

Zastosowanie

Quality Control Testing

Evaluated by Western Blotting in lysate from HepG2 cells treated with IFN and TNF .

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected FAT10 in lysate from HepG2 cells treated with IFN and TNF (200 and 400 units, respectively for 16 h), but not in lysate from untreated HepG2 cells.

Tested Applications

Immunocytochemistry Analysis: A 1:50 dilution from a representative lot detected FAT10/Ubiquitin D in HepG2 cells.

Western Blotting Analysis: A representative lot detected FAT10/Ubiquitin D in Western Blotting application (Aichem, A., et al. (2012). J Cell Sci. 125(Pt19):4576-85; Aichem, A., et al. (2010). Nat Commun.;1:13).

Immunocytochemistry Analysis: A representative lot detected FAT10/Ubiquitin D in Immunocytochemistry applications (Aichem, A., et al. (2012). J Cell Sci. 125(Pt19):4576-85).

Immunoprecipitation Analysis: A representative lot immunoprecipitated FAT10/Ubiquitin D in Immunoprcipitation applications (Aichem, A., et al. (2012). J Cell Sci. 125(Pt19):4576-85).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Opis wartości docelowych

Ubiquitin D (UniProt: O15205; also known as Diubiquitin, UBD, Ubiquitin-like protein FAT10) is encoded by the UBD (also known as FAT10) gene (Gene ID: 10537) in human. FAT10 is a ubiquitin-like modifier protein that is involved in various cellular processes, including apoptosis, cell cycle control, and NF- B activation. It is constitutively expressed in mature dendritic cells and B-cells. Its expression is observed in the reticuloendothelial system, the gastrointestinal system, kidney, lung, and prostate gland. It is detected in the nucleus as well as cytoplasm. It has two ubiquitin-like domains (aa 6-81 and 90-163). It can covalently attach to target protein and lead to their degradation by the 26S proteasome, in a NUB1-dependent manner. Its conjugation ability activated by UBA6. It regulates TNF -induced and LPS-mediated activation of NF- B by promoting TNF - mediated proteasomal degradation of I B . It is reported to mediate mitotic non-disjunction and chromosome instability, in long-term in vitro culture and cancers, by abbreviating mitotic phase and impairing the kinetochore localization of mitotic arrest deficient 2 like 1 (MAD2L1) during the prometaphase stage. It mediates apoptosis in a caspase-dependent manner, especially in renal epithelium and tubular cells during renal diseases such as polycystic kidney disease and HIV-associated nephropathy. p62 protein that is involved in autophagy is covalently mono-FAT10ylated at several lysine residues, which leads to proteasomal degradation of p62. Prolonged induction of endogenous FAT10 by pro-inflammatory cytokines leads to a reduction in endogenous p62 levels. Clone 4F1 displays high-affinity for FAT10 and can immunoprecipitate monomeric FAT10 and FAT10-conjugated substrates. (Ref.: Aichem, A., et al. (2012). J. Cell Sci. 125(19); 4576-4585; Ren, J., et al. (2006). J. Biol. Chem. 281(16); 11413-11421; Ross, MJ., et al. (2006). J. Am. Soc. Nephrol. 17(4); 996-1004; Hipp, MS., et al. (2005). Mol. Cell Biol. 25(9); 3483-3491).

Postać fizyczna

Purified mouse monoclonal antibody IgG2c in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Rekonstytucja

1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Przechowywanie i stabilność

Recommended storage: +2°C to +8°C.

Inne uwagi

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 12 - Non Combustible Liquids
• Klasa zagrożenia wodnego (WGK): WGK 1
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable