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Mixed alkyl aryl phosphonate esters as quenched fluorescent activity-based probes for serine proteases.

Mixed alkyl aryl phosphonate esters as quenched fluorescent activity-based probes for serine proteases.

Organic & biomolecular chemistry (2015-01-03)

Sevnur Serim, Philipp Baer, Steven H L Verhelst

要旨

Activity-based probes (ABPs) are powerful tools for the analysis of active enzyme species in whole proteomes, cells or animals. Quenched fluorescent ABPs (qABPs) can be applied for real time imaging, allowing the visualization of dynamic enzyme activation by fluorescent microscopy. Unfortunately, qABPs are only available for a few enzymes. We here describe the design and synthesis of qABPs for serine proteases based on a phosphonate ester scaffold.

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製品内容

Sigma-Aldrich

酢酸, glacial, ACS reagent, ≥99.7%

Sigma-Aldrich

酢酸, glacial, ReagentPlus^®, ≥99%

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硫酸マグネシウム, anhydrous, ReagentPlus^®, ≥99.5%

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2-ブタノン, ACS reagent, ≥99.0%

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硫酸マグネシウム, anhydrous, reagent grade, ≥97%

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酢酸, glacial, ≥99.99% trace metals basis

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硫酸マグネシウム, BioReagent, suitable for cell culture, suitable for insect cell culture

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USP

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