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由来生物
Bacillus sp. (B. stearothermophilus)
品質水準
タイプ
Type VIII
形状
lyophilized powder
比活性
100-300 units/mg protein
保管温度
2-8°C
詳細
Research Area: CELL SIGNALING
Mammalian pyruvate kinase is a tetrameric protein composed of identical subunits organized in a dimer-of-dimers configuration. Four isoforms of pyruvate kinase exist in mammals, namely PKM1, PKM2, PKR, and PKL. Pyruvate kinase can be found in both tetrameric and dimeric forms.
Mammalian pyruvate kinase is a tetrameric protein composed of identical subunits organized in a dimer-of-dimers configuration. Four isoforms of pyruvate kinase exist in mammals, namely PKM1, PKM2, PKR, and PKL. Pyruvate kinase can be found in both tetrameric and dimeric forms.
アプリケーション
Pyruvate Kinase from Bacillus stearothermophilus may be used in biofuel production.
Pyruvate kinase from Bacillus stearothermophilus has been used in a study to assess evidence that the genes for phosphofructokinase and pyruvate kinase constitute an operon. It has also been used in a study to investigate the importance of the Lys221 active site for pyruvate kinase activity.
生物化学的／生理学的作用
Pyruvate Kinase from Bacillus stearothermophilus is activated by AMP and ribose 5-phosphate.
Pyruvate kinase is an enzyme that facilitates the conversion of phosphoenolpyruvate (PEP) and ADP to pyruvate and ATP in glycolysis and is involved in the regulation of cell metabolism. It serves as a terminal enzyme in the glycolytic pathway. This reaction is favorable due to the high energy of hydrolysis of PEP.
Pyruvate kinase is a key regulator controlling metabolic flux and ATP production in glycolysis and is considered a potential drug target. Additionally, pyruvate kinases are subject to regulation by heterotropic effectors, with fructose 1,6-bisphosphate (FBP) being the most widely known allosteric activator of bacterial, yeast, and mammalian enzymes. Furthermore, PKM2, one of the four isoforms of pyruvate kinase, is extensively expressed in various types of tumors and is associated with tumorigenesis.
Pyruvate kinase is a key regulator controlling metabolic flux and ATP production in glycolysis and is considered a potential drug target. Additionally, pyruvate kinases are subject to regulation by heterotropic effectors, with fructose 1,6-bisphosphate (FBP) being the most widely known allosteric activator of bacterial, yeast, and mammalian enzymes. Furthermore, PKM2, one of the four isoforms of pyruvate kinase, is extensively expressed in various types of tumors and is associated with tumorigenesis.
単位の定義
1 unitは、pH 7.2、30°C、1分間で、1.0 μmoleのホスホ(エノール)ピルビン酸をピルビン酸に変換します。
物理的形状
凍結乾燥品(Trisバッファ-塩含有, pH 8.5)
アナリシスノート
タンパク質はBiuret法で測定
保管分類コード
11 - Combustible Solids
WGK
WGK 3
引火点(°F)
Not applicable
引火点(℃)
Not applicable
個人用保護具 (PPE)
Eyeshields, Gloves, type N95 (US)
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
P1903-100UN:
P1903-500UN:
P1903-2.5KU:
P1903-VAR:
P1903-BULK:
P1903-1KU:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
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E W Walters et al.
Plant physiology, 114(2), 549-555 (1997-06-01)
Adenylosuccinate synthetase (AdSS) is the site of action hydantocidin, a potent microbial phytotoxin. A kinetic analysis of the mode of inhibition of a plant adenylosuccinate synthetase by the active metabolite 5'-phosphohydantocidin (5'-PH) was the objective of the present study. AdSS
Molecular cloning and nucleotide sequence of the gene for pyruvate kinase of Bacillus stearothermophilus and the production of the enzyme in Escherichia coli. Evidence that the genes for phosphofructokinase and pyruvate kinase constitute an operon
Sakai, H. and T. Ohta
FEBS Journal, 211, 851-859 (1993)
Crystallization and preliminary X-ray analysis of pyruvate kinase from Bacillus stearothermophilus
Suzuki, K.
Acta Crystallographica Section B, Structural Crystallography and Crystal Chemistry, F61, 759-761 (2005)
Mutagenesis of the active site lysine 221 of the pyruvate kinase from Bacillus stearothermophilus
Sakai, H.
The Journal of Biological Chemistry, 137, 141-145 (2005)
Nicolas Galazis et al.
European journal of endocrinology, 168(2), R33-R43 (2012-10-25)
Women with polycystic ovary syndrome (PCOS) are at increased risk of developing insulin resistance and type 2 diabetes mellitus (T2DM). In this study, we attempted to list the proteomic biomarkers of PCOS and T2DM that have been published in the
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