P4105

ピルビン酸 オキシダーゼ from Aerococcus sp.

lyophilized powder, ≥35 units/mg protein (biuret)

• 別名: ピルビン酸:酸素オキシドレダクターゼ(リン酸化)
• CAS番号: 9001-96-1
• Enzyme Commission number: 1.2.3.3 (BRENDA, IUBMB)
• EC Number: 232-644-5
• MDL番号: MFCD00132148
• UNSPSCコード: 12352204
• NACRES: NA.54

特性

• 由来生物: bacterial (Aerococcus spp.)
• 品質水準: 200
• 形状: lyophilized powder
• 比活性: ≥35 units/mg protein (biuret)
• 保管温度: −20°C

詳細

アプリケーション

Pyruvate Oxidase (PoxB) converts pyruvate directly to acetate and CO₂. It is used to study pyruvate metabolism. It is used to study aerobic metabolism of bacterium, such as Lactobacillus plantarumand Streptococcus pneumoniae.

Pyruvate Oxidase from Aerococcus sp. has been used in the amplex Red-based fluorescence assay for pyruvate. It is suitable for use in the preparation of biosensor for determination of thiamine (vitamin B1), and as a component in the constant part of the multi-enzyme biocatalytic cascade for the determination of biomarkers for traumatic brain injury (TBI) and soft tissue injury (STI).

生物化学的／生理学的作用

Pyruvate Oxidase consists of four subunits with identical molecular weights. PoxB reacts with certain aldehydes and phosphate can be replaced by arsenate. Oxygen as well as several artificial compounds can function as electron acceptors. Pyruvate Oxidase is activated by phospholipids as well as monomeric and micellar amphiphiles.

Pyruvate Oxidase oxidises pyruvate to form acetate, hydrogen peroxide and carbon dioxide. Pyruvate oxidase requires flavin adenine dinucleotide (FAD), thiamine pyrophosphate (TPP) and magnesium as cofactors for its catalytic activity. Thiamine activates pyruvate oxidase activity.

単位の定義

1ユニットは、pH 6.7、37°Cでピルビン酸とリン酸がアセチルリン酸とCO₂へと変換される反応において、1分間で、1.0 μmolのH₂O₂を生成する酵素量です。

物理的形状

凍結乾燥品(バッファ-塩と糖を含有)

安全性情報

• ピクトグラム: GHS08
• シグナルワード: Danger
• 危険有害性情報: H334
• 注意書き: P261 - P284 - P501
• 危険有害性の分類: Resp. Sens. 1
• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable
• 個人用保護具 (PPE): Eyeshields, Gloves, type N95 (US)