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Merck

71085

Sigma-Aldrich

KOD DNA Polymerase

High fidelity DNA polymerase designed for accurate PCR amplification of DNA templates for general cloning and cDNA amplification applications.

別名:

High fidelity PCR, High fidelity polymerase, KOD polymerase

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About This Item

UNSPSCコード:
41106314
NACRES:
NA.54

リコンビナント

expressed in E. coli

品質水準

アッセイ

≥90% (homogenous band, SDS-PAGE)

使用法

sufficient for 250 reactions

5′ exonuclease activity

<2%, 74 °C (1 h incubation in a reaction with 5′-labeled λ/Sca I digest, per unit enzyme)

secondary activity

nicking (none detected)

特徴

Difficult Templates/Specialty Enzymes PCR
High Fidelity PCR
dNTPs included
hotstart: no

メーカー/製品名

Novagen®

保管条件

OK to freeze

濃度

2.5 unit/μL

テクニック

PCR: suitable

入力

purified DNA

適合性

suitable for PCR

輸送温度

wet ice

保管温度

−20°C

遺伝子情報

Thermococcus kodakaraensis ... TK_RS00010(3233723)

詳細

PCR involves replication of a DNA template by a thermostable DNA polymerase. The processivity, specificity, and fidelity of the polymerase enzyme used can influence the efficiency, reproducibility, and yield of the PCR reaction. High-fidelity PCR, utilizes a DNA polymerase with a low error rate and results in a high degree of accuracy in the replication of the DNA of interest. Fidelity is critical when accurate sequence amplification of the gene target is needed, for example, when direct sequencing or cloning for downstream protein expression. Unwarranted mutation could severely impact your studies.

Our analysis has shown that KOD enzymes are an easy choice for fast, accurate and high-yielding PCR. Our molecular biologists work to develop and formulate polymerases offering the highest specificity, fidelity and yield during PCR amplification. In addition, optimized buffer compositions, convenient master mixes and cycling parameters provide additional ease of use and data reproducibility.KOD DNA Polymerase (formerly KOD HiFi DNA Polymerase) is a recombinant form of Thermococcus kodakaraensis KOD1 DNA polymerase. KOD is a high fidelity thermostable DNA polymerase that amplifies target DNA up to 6 kbp with superior accuracy and yield for PCR applications. The enzyme′s 3′→5′ exonuclease-dependent proofreading activity results in a lower PCR mutation frequency than any other commercially available DNA polymerase. The elongation rate and processivity are 5 times and 10 to 15 times higher, respectively, than for Pfu DNA polymerase, resulting in highly accurate and robust yield, in a short reaction time. The enzyme generates blunt-ended PCR products suitable for cloning with the Novagen Perfectly Blunt® and LIC Vector Kits.

アプリケーション

KOD DNA Polymerase has been used in high fidelity polymerase chain reaction (PCR) to amplify desired severe acute respiratory syndrome coronavirus 2(SARS-CoV-2) N constructs.

特徴および利点

  • Higher fidelity than Pfu DNA polymerase excellent for cloning
  • Greater yield extension speed is 2X faster than Taq DNA polymerase and 5X faster than Pfu DNA polymerase
  • Higher processivity sequential nucleotide polymerization is 10- to 15-fold greater than Pfu and Tli DNA polymerases
  • Amplifies plasmid and lambda DNA templates up to 6 kbp
  • Amplifies genomic DNA templates up to 2 kbp
  • No truncated amplification products

構成

  • 250 U KOD DNA Polymerase (2.5 U/μl)
  • 1 ml 10X Buffer #1 for KOD DNA Polymerase (pH 8.0)
  • 1 ml 10X Buffer #2 for KOD DNA Polymerase (pH 8.8)
  • 1 ml 25 mM MgCl
  • 1 ml dNTP Mix (2 mM each)

警告

Toxicity: Standard Handling (A)

単位の定義

One unit is defined as the amount of enzyme that will catalyze the incorporation of 10 nmol of dNTP into acid-insoluble form in 30 min at 75°C, in a reaction containing 20 mM Tris-HCl (pH 7.5 at 25°C), 8 mM MgCl₂, 7.5 mM DTT, 50 µg/ml BSA, 150 µM each of dATP, dCTP, dGTP, dTTP (a mix of unlabeled and [3H]dTTP) and 150 µg/ml activated calf thymus DNA.

法的情報

Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser′s own internal research. No other patents rights (such as 5′ Nuclease Process patent rights) are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California, 94404, USA.
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
Perfectly Blunt is a registered trademark of Merck KGaA, Darmstadt, Germany

ピクトグラム

Exclamation mark

シグナルワード

Warning

危険有害性情報

危険有害性の分類

Aquatic Chronic 3 - Eye Irrit. 2

保管分類コード

10 - Combustible liquids

WGK

WGK 2


適用法令

試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。

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試験成績書(COA)

製品のロット番号・バッチ番号を入力して、試験成績書(COA) を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。

以前この製品を購入いただいたことがある場合

文書ライブラリで、最近購入した製品の文書を検索できます。

文書ライブラリにアクセスする

M Takagi et al.
Applied and environmental microbiology, 63(11), 4504-4510 (1997-11-15)
The DNA polymerase gene from the archaeon Pyrococcus sp. strain KOD1 (KOD DNA polymerase) contains a long open reading frame of 5,013 bases that encodes 1,671 amino acid residues (GenBank accession no. D29671). Similarity analysis revealed that the DNA polymerase
Daisuke Miki et al.
Methods in molecular biology (Clifton, N.J.), 2200, 121-146 (2020-11-12)
CRISPR/Cas9 system has emerged as a powerful genome engineering tool to study gene function and improve plant traits. Genome editing is achieved at a specific genome sequence by Cas9 endonuclease to generate double standard breaks (DSBs) directed by short guide
Damien Jacot et al.
Methods in molecular biology (Clifton, N.J.), 2071, 125-141 (2019-11-24)
The phylum Apicomplexa groups numerous pathogenic protozoan parasites including Plasmodium, the causative agent of malaria, Cryptosporidium which can cause severe gastrointestinal infections, as well as Babesia, Eimeria, and Theileria that account for considerable economic burdens to poultry and cattle industry.
Luca Murer et al.
Journal of virology, 96(2), e0106021-e0106021 (2021-10-28)
Rhinoviruses (RVs) cause recurrent infections of the nasal and pulmonary tracts, life-threatening conditions in chronic respiratory illness patients, predisposition of children to asthmatic exacerbation, and large economic cost. RVs are difficult to treat. They rapidly evolve resistance and are genetically
Chao Wu et al.
STAR protocols, 2(4), 100906-100906 (2021-10-14)
Nucleocapsid proteins are essential for SARS-CoV-2 life cycle. Here, we describe protocols to gather domain-specific insights about essential properties of nucleocapsids. These assays include dynamic light scattering to characterize oligomerization, fluorescence polarization to quantify RNA binding, hydrogen-deuterium exchange mass spectrometry

資料

The purpose of Hot Start PCR is to inhibit the PCR reaction in order to reduce nonspecific amplification, prevent the formation of primer dimers, and increase product yields.

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