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Merck

KMM-201NV

Sigma-Aldrich

KOD One PCR Master Mix -BLUE-

Ready-to-use 2X hot-start PCR master mix with a modified KOD DNA polymerase optimized for ultra-fast and convenient high-fidelity PCR

別名:

Fast Hot-start PCR, High-fidelity DNA polymerase, KOD One PCR Ready Mix

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About This Item

UNSPSCコード:
41106314
NACRES:
NA.55

由来生物

Escherichia coli (carrying the cloned UKOD DNA polymerase gene)

品質水準

形状

liquid

使用法

sufficient for 25 reactions
sufficient for 500 reactions

特徴

Fast PCR
High Fidelity PCR
dNTPs included
hotstart

テクニック

PCR: suitable

入力

crude DNA

輸送温度

dry ice

保管温度

−20°C

詳細

KOD One PCR master Mix -Blue- is a ready-to-use 2 x PCR master mix containing a novel genetically modified KOD DNA polymerase (UKOD) along with a new elongation accelerator, enabling fast PCR with an extension time of 5 sec/ kb for template DNA <10kb. This master mix has greater efficiency and flexibility than conventional PCR enzymes with an ability to amplify from crude specimens such as blood and mouse cell lysates. In addition to crude samples, it can be used on templates containing uracils (dU). The master mix can use primers containing both inosines (dI) and dU for amplification.

This hot start KOD polymerase is antibody-inactivated with two types of antibodies that prevent the 5′→3′ polymerase activity and 3′→5′ exonuclease activity. Upon heat-activation, the 3′→5′ proof-reading ability of the enzyme generates blunt-end PCR products.

アプリケーション

  • Genomic DNA amplification
  • cDNA amplification
  • Direct PCR
  • Colony PCR
  • Amplification of NGS libraries
  • Site direct gene mutation

Compatible Sample Types: Serum, Plasma, Cell, Plant, soil extraction, DNA, nail, hair etc.
Sample Volume Needed: about 50ng DNA

特徴および利点

  • Fast: Amplifies the targets using the following very short conditions:
o =1 kb: 1 sec
o 1∼ 10 kb: 5 sec/ kb
o 10 kb∼: 10 sec/ kb
  • Ready-to-use: 2X premixed solution of UKOD DNA polymerase, buffer, dNTPs, and MgCl2. KOD One PCR Master Mix Blue includes a loading dye (BPB) to allow direct loading onto agarose gels.
  • High Fidelity: Approximately 80-fold higher fidelity than conventional Taq DNA polymerase. This mix can be used for various purposes where this would be an advantage (e.g., in the preparation of long target amplicons for sequencing).
  • High Efficiency: Enables high throughput PCR and ultra-fast PCR cycling conditions with an extension time of as quick as 5 sec/kb.
  • Crude Sample PCR: Effective for direct PCR amplification of crude samples such as biological samples, food samples, soil extract, etc.
  • High flexibility: Amplifies templates containing uracils (dU). Also, amplifies fragments using degenerate primers with inosines (dI) and uracils (dU)

包装

KMM-201NV - 5X1ML is sufficient for 500 reactions at 20 μL each or for 200 reactions at 50 μL each.
KMM-201NVS - 0.25ML is sufficient for 25 reactions at 20 μL each or for 10 reactions at 50 μL each.

保管および安定性

Stored at -20 °C for a year. 4 °C for a month.

その他情報

For R&D use only. Not for drug, household, or other uses.

法的情報

*Manufactured by Toyobo and distributed by Sigma-Aldrich. Not available in Japan.
KOD One is a trademark of Toyobo Co., Ltd.

ピクトグラム

Corrosion

シグナルワード

Warning

危険有害性情報

注意書き

危険有害性の分類

Met. Corr. 1

保管分類コード

8B - Non-combustible corrosive hazardous materials

WGK

WGK 2

引火点(°F)

Not applicable

引火点(℃)

Not applicable


適用法令

試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。

Jan Code

KMM-201NV-5X1ML:
KMM-201NVS-0.25ML:


試験成績書(COA)

製品のロット番号・バッチ番号を入力して、試験成績書(COA) を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。

以前この製品を購入いただいたことがある場合

文書ライブラリで、最近購入した製品の文書を検索できます。

文書ライブラリにアクセスする

Moe Yokoshi et al.
Molecular cell, 78(2), 224-235 (2020-02-29)
Formation of self-associating loop domains is a fundamental organizational feature of metazoan genomes. Here, we employed quantitative live-imaging methods to visualize impacts of higher-order chromosome topology on enhancer-promoter communication in developing Drosophila embryos. Evidence is provided that distal enhancers effectively
Nuria Cortes-Silva et al.
Current biology : CB, 30(4), 561-572 (2020-02-08)
Accurate chromosome segregation requires assembly of the multiprotein kinetochore complex at centromeres. In most eukaryotes, kinetochore assembly is primed by the histone H3 variant CenH3 (also called CENP-A), which physically interacts with components of the inner kinetochore constitutive centromere-associated network

関連コンテンツ

KOD One™ PCR Master Mix overview for ultra-fast PCR with high specificity, fidelity, and yield

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