KMM-101NV
KOD One™ PCR Master Mix
Ready-to-use 2X hot-start PCR master mix with a modified KOD DNA polymerase optimized for ultra-fast and convenient high-fidelity PCR
別名:
Fast Hot-start PCR, High-fidelity DNA polymerase, KOD One™ PCR Ready Mix
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About This Item
UNSPSCコード:
41106314
NACRES:
NA.55
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詳細
KOD One™ PCR master Mix is a ready-to-use 2 x PCR master mix containing a novel genetically modified KOD DNA polymerase (UKOD) along with a new elongation accelerator, enabling fast PCR with an extension time of 5 sec/kb for template DNA <10kb. This master mix has greater efficiency and flexibility than conventional PCR enzymes with an ability to amplify from crude specimens such as blood and mouse cell lysates. In addition to crude samples, it can be used on templates containing uracils (dU). The master mix can use primers containing both inosines (dI) and dU for amplification.
This hot start KOD polymerase is antibody-inactivated with two types of antibodies that prevent the 5′→3′ polymerase activity and 3′→5′ exonuclease activity. Upon heat-activation, the 3′→5′ proof-reading ability of the enzyme generates blunt-end PCR products.
This hot start KOD polymerase is antibody-inactivated with two types of antibodies that prevent the 5′→3′ polymerase activity and 3′→5′ exonuclease activity. Upon heat-activation, the 3′→5′ proof-reading ability of the enzyme generates blunt-end PCR products.
アプリケーション
- Genomic DNA amplification
- cDNA amplification
- Direct PCR
- Colony PCR
- Amplification of NGS libraries
- Site direct gene mutation
Compatible Sample Types: Serum, Plasma, Cell, Plant, soil extraction, DNA, nail, hair etc.
Sample Volume Needed: about 50ng DNA
特徴および利点
- Fast: Amplifies the targets using the following very short conditions:
o 1~ 10 kb: 5 sec/ kb
o 10 kb~: 10 sec/ kb
- Ready-to-use: 2X premixed solution of UKOD DNA polymerase, buffer, dNTPs, and MgCl2.
- High Fidelity: Approximately 80-fold higher fidelity than conventional Taq DNA polymerase
- High Efficiency: Enables high throughput PCR and ultra-fast PCR cycling conditions with an extension time of as quick as 5 sec/kb.
- Crude Sample PCR: Effective for direct PCR amplification of crude samples such as biological samples, food samples, soil extract, etc.
- High flexibility: Amplifies templates containing uracils (dU). Also, amplifies fragments using degenerate primers with inosines (dI) and uracils (dU)
包装
KMM-101NV - 5X1ML is sufficient for 500 reactions at 20 μL each or for 200 reactions at 50 μL each.
KMM-101NVS - 0.25ML is sufficient for 25 reactions at 20 μL each or for 10 reactions at 50 μL each.
KMM-101NVS - 0.25ML is sufficient for 25 reactions at 20 μL each or for 10 reactions at 50 μL each.
その他情報
For R&D use only. Not for drug, household, or other uses.
法的情報
*Manufactured by Toyobo and distributed by Sigma-Aldrich. Not available in Japan.
KOD One is a trademark of Toyobo Co., Ltd.
シグナルワード
Warning
危険有害性情報
注意書き
危険有害性の分類
Met. Corr. 1
保管分類コード
8B - Non-combustible corrosive hazardous materials
WGK
WGK 2
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
KMM-101NV-5X1ML:
KMM-101NVS-0.25ML:
最新バージョンのいずれかを選択してください：
Moe Yokoshi et al.
Molecular cell, 78(2), 224-235 (2020-02-29)
Formation of self-associating loop domains is a fundamental organizational feature of metazoan genomes. Here, we employed quantitative live-imaging methods to visualize impacts of higher-order chromosome topology on enhancer-promoter communication in developing Drosophila embryos. Evidence is provided that distal enhancers effectively
Nuria Cortes-Silva et al.
Current biology : CB, 30(4), 561-572 (2020-02-08)
Accurate chromosome segregation requires assembly of the multiprotein kinetochore complex at centromeres. In most eukaryotes, kinetochore assembly is primed by the histone H3 variant CenH3 (also called CENP-A), which physically interacts with components of the inner kinetochore constitutive centromere-associated network
関連コンテンツ
KOD One™ PCR Master Mix overview for ultra-fast PCR with high specificity, fidelity, and yield
Global Trade Item Number
| カタログ番号 | GTIN |
|---|---|
| KMM-101NV-5X1ML | 4061841805342 |
| KMM-101NVS-0.25ML | 4061841805359 |
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