R0884

T7 RNA Polymerase

recombinant, expressed in E. coli, buffered aqueous solution

• Sinonimo/i: RNA Polymerase T7, RNA Polymerase, T7 from E. coli HMS 174/pAR1219
• Numero CAS: 9014-24-8
• Classificazione EC (Enzyme Commission): 2.7.7.6 (BRENDA, IUBMB)
• Numero MDL: MFCD00132197
• Codice UNSPSC: 12352204

Proprietà

• Ricombinante: expressed in E. coli
• Grado: for molecular biology
• Forma fisica: buffered aqueous solution
• PM: 98.8 kDa
• Concentrazione: 10,000-50,000 U/mL
• N° accesso UniProt: P00573
• Attività estranea: DNase and RNase, none detected
• Temperatura di conservazione: −20°C
• Informazioni sul gene: bacteriophage T7 ... T7p07(1261050)

Descrizione

Descrizione generale

T7 RNA polymerase is highly specific for the bacteriophage T7 promoter and terminator sequences. It is extensively used to prepare RNA transcripts for stuctural and metabolic studies. The RNA transcripts can be converted to probes for sensitive hybridization detection studies. T7 polymerase and dideoxynucleotides can be used to directly sequence DNA.

Componenti

T7 RNA Polymerase is supplied as a solution of 100 mM NaCl, 50 mM Tris-HCl (pH 7.9), 0.1 mM EDTA, 0.1% Triton X-100, 1 mM DTT, and 50% (v/v) glycerol.

Definizione di unità

One unit will catalyze the incorporation of 1 nmol of rNTP into acid-precipitable material in 60 min at 37°C.

Risultati analitici

Activity assay: 40 mM Tris-HCl, pH 7.9, 6 mM MgCl₂, 4 mM spermidine, 10 mM DTT, 0.5 μM each rNTP + 10 μCi α-³²P-UTP, 3-10 units of enzyme, and 1 μg of a 350 bp template are incubated for 10 min at 37°C in a total volume of 100 μl. Typical results are ≥50% incorporation of labeled nucleotide into ≥90% full-length transcript.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 10 - Combustible liquids
• Classe di pericolosità dell'acqua (WGK): WGK 3
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable