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Documenti

P4978

Sigma-Aldrich

Phosphatase, Alkaline from calf intestine

buffered aqueous glycerol solution

Sinonimo/i:

CIAP, CIP, Orthophosphoric-monoester phosphohydrolase (alkaline optimum)

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About This Item

Numero CAS:
9001-78-9
Classificazione EC (Enzyme Commission):
3.1.3.1 (BRENDA, IUBMB)
Numero MDL:
MFCD00131849
Codice UNSPSC:
12352204
eCl@ss:
42010105
NACRES:
NA.53

Grado

for molecular biology

Forma fisica

buffered aqueous glycerol solution

PM

~80 kDa

Concentrazione

≥10,000 units/mL

N° accesso UniProt

P19111

Attività estranea

DNase, RNase, none detected

Condizioni di spedizione

wet ice

Temperatura di conservazione

−20°C

Informazioni sul gene

cow ... ALPI(280993)

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Descrizione generale

Alkaline phosphatase is a phosphomonoesterase that catalyzes the cleavage of terminal phosphates from nucleic acids. The free 5′-OH can be phosphorylated with polynucleotide kinase and γ32P-ATP to produce γ32P-end-labeled nucleic acids. Linearized cloning vectors can be prevented from recircularizing by dephosphorylation with alkaline phosphatase.

Applicazioni

Commonly used to remove the 5′-terminal phosphate from nucleic acids during molecular cloning reactions.

Componenti

Alkaline phosphatase is provided in a solution of 10 mM Tris-HCl (pH 8.2), 50 mM KCl, 1 mM MgCl2, and 0.1 mM ZnCl2, in 50% (w/v) glycerol. 10X CIP Buffer, Product Number C3225, is included.

Definizione di unità

One unit will hydrolyze 1 μmole of p-nitrophenyl phosphate per min at 37 °C.

Altre note

Alkaline phosphatase in 5 mM EDTA (pH 8) will be irreversibly heat inactivated at 75 °C for 10 minutes.

Codice della classe di stoccaggio

10 - Combustible liquids

Classe di pericolosità dell'acqua (WGK)

WGK 1

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Certificati d'analisi (COA)

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