Passa al contenuto
Merck
Tutte le immagini(1)

Key Documents

View All Documentation

79390

Sigma-Aldrich

Phosphatase, Alkaline from calf intestinal mucosa

suitable for enzyme immunoassay, solution (clear, colorless), ~2500 U/mg protein (~10 mg/ml)

Sinonimo/i:

Alkaline Phosphatase from calf intestinal mucosa

Autenticatiper visualizzare i prezzi riservati alla tua organizzazione & contrattuali
Tutte le immagini(1)

About This Item

Numero CAS:
9001-78-9
Classificazione EC (Enzyme Commission):
3.1.3.1 (BRENDA, IUBMB)
Numero CE:
232-631-4
Numero MDL:
MFCD00131849
Codice UNSPSC:
12352204
eCl@ss:
42010105
NACRES:
NA.54

Origine biologica

bovine (calf) intestinal mucosa

Forma fisica

solution (clear, colorless)

Attività specifica

~2500 U/mg protein (~10 mg/ml)

PM

Mr ~140000

tecniche

enzyme immunoassay: suitable

Impurezze

0.1 mM ZnCl2
1 mM MgCl2
3 M NaCl
30 mM triethanolamine

pH

~7.6

Temperatura di conservazione

2-8°C

Cerchi prodotti simili? Visita Guida al confronto tra prodotti

Applicazioni

Alkaline phosphatase is used in the laboratory to remove phosphate monoesters which prevents self-ligation. It is also used in the dairy industry to confirm pasturization of milk. Alkaline phosphatse is commonly conjugated to antibodies and used in immunoblotting, ELISA′s, and other antibody based detection assays.

Azioni biochim/fisiol

Alkaline phosphatase is a hydrolytic enzyme which dephosphorylates a variety of molecules under basic conditions.

Definizione di unità

1 U corresponds to the amount of enzyme which hydrolyzes 1 μmol 4-nitrophenyl phosphate per minute at pH 9.8 and 37 °C

Ricostituzione

The solution can be used without dialysis (for enzyme immuno-assay).

Altre note

Sales restrictions may apply
Used in highly sensitive (pg/m) enzyme immunoassays with LCEC detection of phenol; Comparison of three methods for conjugating alkaline phosphatase to human placental lactogen; Preparation of enzyme-antibody conjugates; Liposome immunoassay with antigen-coupled liposomes containing alkaline phosphatase

Pittogrammi

Health hazard
GHS08

Avvertenze

Danger

Indicazioni di pericolo

H334

Consigli di prudenza

P261 - P284 - P501

Classi di pericolo

Resp. Sens. 1

Codice della classe di stoccaggio

10 - Combustible liquids

Classe di pericolosità dell'acqua (WGK)

WGK 1

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves, type N95 (US)

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

Possiedi già questo prodotto?

I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.

Visita l’Archivio dei documenti

M.I. Halliday et al.
Biochemical Society Transactions, 14, 473-473 (1986)
K.R. Wehrmeyer et al.
Journal of Liquid Chromatography, 6, 2141-2141 (1983)
D G Williams
Journal of immunological methods, 72(1), 261-268 (1984-08-03)
One of the requirements for enzyme immunoassay is the formation of a labelled component of the assay system which can be either the ligand or the binder. Three methods for conjugating alkaline phosphatase to human placental lactogen were investigated, involving
Enzyme-linked immunosorbent assay, Elisa. 3. Quantitation of specific antibodies by enzyme-labeled anti-immunoglobulin in antigen-coated tubes.
E Engvall et al.
Journal of immunology (Baltimore, Md. : 1950), 109(1), 129-135 (1972-07-01)
C K Kim et al.
Journal of immunological methods, 159(1-2), 101-106 (1993-02-26)
Immunoliposomes were prepared and their immunoassay applications investigated. Liposomes were prepared from cholesterol and phospholipids including maleimidobenzoylphosphatidylethanolamine (MBPE) for conjunction with thiol-containing antigens. Alkaline phosphatase (APase) was entrapped in the liposome and BSA, the antigen, was modified by reaction with
Visualizza tutti i documenti correlati

Il team dei nostri ricercatori vanta grande esperienza in tutte le aree della ricerca quali Life Science, scienza dei materiali, sintesi chimica, cromatografia, discipline analitiche, ecc..

Contatta l'Assistenza Tecnica.