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Key Documents

10745782001

Roche

DNA, lambda

from bacteriophage lambda cl857 Sam 7

Sinonimo/i:

lambda dna

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About This Item

Codice UNSPSC:
41106310

Livello qualitativo

Forma fisica

solution

PM

32,300 kDa

Confezionamento

pkg of 1 mL

Produttore/marchio commerciale

Roche

Condizioni di stoccaggio

avoid repeated freeze/thaw cycles

Concentrazione

250 μg/mL

Impurezze

RNA, none detected
nuclease, none detected

Colore

colorless

Solubilità

water: miscible

Condizioni di spedizione

dry ice

Temperatura di conservazione

−20°C (−15°C to −25°C)

Descrizione generale

The DNA of bacteriophage lambda contains 48,502 nucleotide pairs forming a linear duplex. The 5′ ends of this DNA molecule have self-complementary single-stranded protrusions of 12 nucleotides that can anneal to form circles. Upon entry into the host cell, the DNA circularizes in the presence of DNA ligase. Many such circles are generated during the early lytic infection. At a later stage in replication, these individual circles/rings are converted to linear concatemers by a process called rolling-circle replication. The linear concatemers are cleaved during packaging to form monomeric chromosomal units by the enzyme DNA terminase.

Applicazioni

DNA, lambda has been used to spike peripheral blood leukocytes (PBL) before genomic DNA isolation. It has also been used in the preparation of biotinylated double-stranded DNA (dsDNA) substrate.

Qualità

The DNA shows the typical cleavage pattern for wild type λDNA in gel electrophoresis after cleavage with restriction endonucleases EcoR I and Hind III.

Sequenza

Chain Length 48,502 bp

Stato fisico

Solution, 250 μg/ml, in 10 mM Tris-HCl, 1 mM EDTA, pH 8.0, ready-to-use

Stoccaggio e stabilità

Store at -15 to -25°C until use; once thawed, store at 2-8 °C.

Altre note

For life science research only. Not for use in diagnostic procedures.

Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

nwg

Punto d’infiammabilità (°F)

No data available

Punto d’infiammabilità (°C)

No data available


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