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Key Documents

10165948001

Roche

RNA, MS2

from bacteriophage MS2

Sinonimo/i:

RNA, ribonucleic acid

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About This Item

Codice UNSPSC:
41106311

Origine biologica

bacteriophage MS2

Livello qualitativo

Forma fisica

solution

PM

~1200 kDa

Confezionamento

pkg of 500 μL (10 A260 units)

Produttore/marchio commerciale

Roche

tecniche

RNA extraction: suitable

Colore

colorless

Solubilità

water: miscible

Temperatura di conservazione

−20°C (−15°C to −25°C)

Categorie correlate

Descrizione generale

RNA, from bacteriophage MS2 is composed of 3569 nucleotides. MS2 bacteriophage acts as a model system in studying molecular biology processes, such as viral RNA replication, process of translation and physiology of infected cells. MS2 RNA codes for three viral polypeptides namely, protein A, coat protein and RNA replicase complex. Protein A and coat protein make up the structure of MS2 virus.

Applicazioni

RNA, MS2 has been used:
  • as a standard to assess RNA quantification methods
  • in RNA extraction
  • in single-tube, fluorescent PERT assay (STF-PERT) for accurate quantitation of different retrovirus types
  • for studies which use natural RNA in a in vivo and in vitro protein-synthesizing system, initiation, elongation, and termination of polypeptide synthesis.
  • for structural and functional studies.
  • to stabilize RNA during cDNA synthesis
  • to improve RNA yield during RNA extraction or isolation

Qualità

Typical analysis: Free of protein and host nucleic acids according to current Quality Control procedures.

Sequenza

Chain Length 3,569 nucleotides

Nota sulla preparazione

Working solution: Storage buffer: 10 mM Tris-HCl, 1 mM EDTA, pH 7.0.

Altre note

For life science research only. Not for use in diagnostic procedures.

Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

nwg

Punto d’infiammabilità (°F)

No data available

Punto d’infiammabilità (°C)

No data available


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Rom S Leidner et al.
PloS one, 8(3), e57841-e57841 (2013-03-09)
Genome-wide platforms for high-throughput profiling of circulating miRNA (oligoarray or miR-Seq) offer enormous promise for agnostic discovery of circulating miRNA biomarkers as a pathway for development in breast cancer detection. By harmonizing data from 15 previous reports, we found widespread
Thomas C Roberts et al.
Biological procedures online, 16(1), 5-5 (2014-03-19)
MicroRNAs (miRNAs) are short RNA molecules which regulate gene expression in eukaryotic cells, and are abundant and stable in biofluids such as blood serum and plasma. As such, there has been heightened interest in the utility of extracellular miRNAs as
Roman Aranda et al.
Analytical biochemistry, 387(1), 122-127 (2009-05-21)
Quantification of RNA is essential for various molecular biology studies. In this work, three quantification methods were evaluated: ultraviolet (UV) absorbance, microcapillary electrophoresis (MCE), and fluorescence-based quantification. Viral, bacterial, and eukaryotic RNA were measured in the 500 to 0.05-ng microl(-1)
Michael Eikmans et al.
International journal of molecular sciences, 21(11) (2020-06-12)
About 10-15% of couples who want to conceive suffer from subfertility, while in 30% of these cases, a male factor plays a role. Levels of particular microRNAs in seminal plasma, including those involved in spermatogenesis, may serve as an indicative
Shalini Das Gupta et al.
Scientific reports, 10(1), 9012-9012 (2020-06-04)
Quantification of plasma microRNAs (miRNAs) as non-invasive disease biomarkers is subject to multiple technical variabilities. This study aimed to develop an optimized protocol for miRNA quantification from rodent plasma. We hypothesized that a fixed small RNA concentration input for reverse

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