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10108626001

Roche

Poly(A)

lyophilized, suitable for PCR, pkg of 100 mg

Sinonimo/i:

Poly(A), Polyadenylic acid

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About This Item

Codice UNSPSC:
41105600

Descrizione

Polyadenylic acid

Livello qualitativo

100

Forma fisica

lyophilized

PM

700-3500 kDa

Confezionamento

pkg of 100 mg

Produttore/marchio commerciale

Roche

Concentrazione

0.5 mg/mL (Working concentration)

tecniche

PCR: suitable

Colore

white

Solubilità

water: soluble

Rapporto di assorbanza

A290/260 nm 0.03-0.05
A280/260 nm 0.28-0.32
A250/260 nm 0.86-0.90

Temperatura di conservazione

2-8°C

Descrizione generale

Poly(A) is used as a carrier for quantitative precipitation of DNA and RNA.

Applicazioni

Poly(A) has been used for droplet digital PCR (ddPCR) assay.
Polyadenylic acid (Poly(A)) to inhibit Exo1′s exonuclease activity. It has also been used as a carrier to resuspend lyophilized gBlocks.

Azioni biochim/fisiol

Polyadenylic acid (Poly(A)) tails present at 3′ end are produced in the cell nucleus. It contains ~250 nucleotides in mammalian cells. Poly(A) regulates mRNA decay and the initiation of translation. Cytoplasmic poly(A) extension modulates translation.

Qualità

Typical analysis:
2.3μmol/mg Poly(A) (from absorbance) in relation to one mononucleotide unit. Chromatographically homogeneous.
Absorbance determination A250/A260, A280/A260, A290/A260

Sequenza

Chain Length 2.100 to 10.000 nucleotides

Definizione di unità

1 A260 unit corresponds to 40 μg ssRNA.

Stato fisico

Lyophilizate, potassium salt

Nota sulla preparazione

Working concentration: 0.5 mg/ml
A concentration of 0.5 mg/ml is recommended.
Working solution: 0.5 mg/ml is recommended.

Altre note

For life science research only. Not for use in diagnostic procedures.

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 1

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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Poly (A) tail length is controlled by the nuclear poly (A)-binding protein regulating the interaction between poly (A) polymerase and the cleavage and polyadenylation specificity factor
Kuhn U, et al.
The Journal of Biological Chemistry, 284(34), 22803-22814 (2009)
Yuichiro Miyaoka et al.
Scientific reports, 6, 23549-23549 (2016-04-01)
Precise genome-editing relies on the repair of sequence-specific nuclease-induced DNA nicking or double-strand breaks (DSBs) by homology-directed repair (HDR). However, nonhomologous end-joining (NHEJ), an error-prone repair, acts concurrently, reducing the rate of high-fidelity edits. The identification of genome-editing conditions that
Poly (ADP-ribose)-binding promotes Exo1 damage recruitment and suppresses its nuclease activities
Cheruiyot A, et al.
DNA Repair, 35, 106-115 (2015)
Peiguo Yang et al.
Cell, 181(2), 325-345 (2020-04-18)
The mechanisms underlying ribonucleoprotein (RNP) granule assembly, including the basis for establishing and maintaining RNP granules with distinct composition, are unknown. One prominent type of RNP granule is the stress granule (SG), a dynamic and reversible cytoplasmic assembly formed in

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