R1158
Ribonuclease Inhibitor
recombinant, expressed in E. coli, 20-40 units/μL
Sinonimo/i:
RNAse inhibitor
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About This Item
Prodotti consigliati
Ricombinante
expressed in E. coli
Livello qualitativo
Grado
for molecular biology
Forma fisica
buffered aqueous solution
PM
~50 kDa
Concentrazione
20-40 units/μL
Condizioni di spedizione
dry ice
Temperatura di conservazione
−20°C
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Descrizione generale
Ribonuclease inhibitor works to inhibit RNase activity by forming a tight, non-covalent 1:1 complex. It is derived from E. coli which expresses portions of the human placental ribonuclease inhibitor. It inhibits RNases A, B, and C. It will not inhibit RNases H, 1, T1, S1 Nuclease, SP6, T7 RNA Polymerase, T3 RNA Polymerase, AMV Reverse Transcriptase, M-MLV Reverse Transcriptase, or Taq Polymerase. The inhibitor can be removed by phenol extraction or by heating to 65°C for 10 minutes. The pH range for inhibition is pH 5.5–9 (highestinhibition at pH 7–8).
Applicazioni
Ribonuclease Inhibitor is suitable for use in useful for in vitro inhibition of ribonucleases, including procedures like:
- cDNA synthesis from mRNA
- reverse transcriptase polymerase chain reaction (RT-PCR)
- in vitro transcription and translation
- mRNA protection from degradation
- formaldehyde cross-linked lncRNA purification
- RNA-fluorescence in situ hybridization (FISH)
Useful for in vitro inhibition of ribonucleases, including procedures like cDNA synthesis, RT-PCR, and in vitro transcription and translation.
Azioni biochim/fisiol
Inhibits RNase by forming a tight, non-covalent 1:1 complex.
Componenti
Ribonuclease Inhibitor is provided as a solution in 20 mM HEPES-KOH( pH 7.6), 50 mM KCl, 8 mM DTT and 50% glycerol.
Definizione di unità
One unit will reduce the activity of 5 ng of ribonuclease A by 50% in a cytidine 2′:3′-cyclic monophosphate system.
Stato fisico
Solution in 20 mM HEPES-KOH, pH 7.6, 50 mM KCl, 8 mM DTT and 50% glycerol.
Altre note
- Denaturing conditions (i.e., urea or temperatures ≥50 °C) should be avoided as they may cause a release of active ribonuclease from the complex.
- This product is for R&D use only, not for drug, household, or other uses.
Prodotti correlati
N° Catalogo
Descrizione
Determinazione del prezzo
Avvertenze
Danger
Indicazioni di pericolo
Consigli di prudenza
Classi di pericolo
Eye Dam. 1
Codice della classe di stoccaggio
10 - Combustible liquids
Classe di pericolosità dell'acqua (WGK)
WGK 3
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Certificati d'analisi (COA)
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