R7876

Ribonucleic acid, transfer from wheat germ

Type V, 15-19 units/mg solid

• Sinonimo/i: Transfer RNA, tRNA
• Numero CAS: 9014-25-9
• Numero MDL: MFCD00132200
• Codice UNSPSC: 12352106
• NACRES: NA.55

Proprietà

• Grado: for molecular biology
• Livello qualitativo: 300
• Tipo: Type V
• Forma fisica: lyophilized powder
• Attività specifica: 15-19 units/mg solid
• Condizioni di stoccaggio: desiccated
• Solubilità: H₂O: ~2 mg/mL, clear to hazy
• Temperatura di conservazione: 2-8°C

Descrizione

Descrizione generale

Wheat germ tRNA contains many different specific tRNA molecules.

Applicazioni

Crude transfer RNA (tRNA), purified from wheat germ, contains many specific tRNA molecules that can be separated by different processes. Experiments for detailed investigation of structure and function using in vitro translation systems often require highly purified tRNA, which can be achieved by combinations of chromatographic separations and reversible biochemical modification of the tRNA. Wheat germ tRNA has been used for the purification of RNAse Z (tRNA 3′- processing endonucleases) from wheat germ. The purification included six steps with the most efficient one using an affinity column made of wheat germ tRNA, which the RNAse Z bound tightly.

Suitable for use as a carrier in nucleic acid purification and precipitation.

Componenti

tRNA is provided as a lyophilized powder that is soluble in water at a concentration of 2 mg/ml.

Definizione di unità

One unit will yield an absorbance of 1.0 at 260 nm in 1.0 mL of water (1 cm light path).

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 3
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable
• Dispositivi di protezione individuale: Eyeshields, Gloves, type N95 (US)