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S6639

Sigma-Aldrich

SSC Buffer 20× Concentrate

for Northern and Southern blotting, solution

Sinonimo/i:

20X SSC Buffer, SSC hybridization buffer, Saline-sodium citrate buffer

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About This Item

Codice UNSPSC:
41105319
NACRES:
NA.25

Grado

for molecular biology

Livello qualitativo

200

Descrizione

Saline-Sodium Citrate buffer, made with ultrapure water

Sterilità

0.2 μm filtered

Forma fisica

solution

applicazioni

diagnostic assay manufacturing

Attività estranea

DNAse, none detected
Endonuclease, none detected
Exonuclease, none detected
NICKase, none detected
RNAse, none detected

Temperatura di conservazione

room temp

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Descrizione generale

SSC (Saline Sodium Citrate) Buffer 20x liquid is a standard reagent in Southern and Northern hybridization procedures. It is used to control stringency of wash buffer for the washing steps after hybridization.

Applicazioni

SSC Buffer 20× Concentrate has been used
  • as a hybridization buffer on in situ hybridization human-specific repetitive Alu sequence
  • as a washing buffer for membrane for Southern hybridization, dot blot of mice genomic DNA and northern blotting of microRNA
  • as an incubation buffer in fluorescence in situ hybridization (FISH) of L1 retrotransposon

Caratteristiche e vantaggi

  • Convenient ready-to-use liquid
  • Multi-purpose use in hybridizations

Componenti

SSC Buffer 20x contains 3M NaCl in 0.3M sodium citrate (pH 7.0).

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 1

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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