H7523

HEPES

BioXtra, pH 5.0-6.5 (1 M in H₂O), ≥99.5% (titration)

• Sinonimo/i: Acido 4-(2-idrossietil)piperazina-1-etansolfonico, N-(2-idrossietil)piperazina-N′-(acido 2-etansolfonico)
• Formula empirica (notazione di Hill): C₈H₁₈N₂O₄S
• Numero CAS: 7365-45-9
• Peso molecolare: 238.30
• Beilstein: 883043
• Numero CE: 230-907-9
• Numero MDL: MFCD00006158
• Codice UNSPSC: 12161700
• ID PubChem: 24895796
• NACRES: NA.25

Proprietà

• Nome Commerciale: BioXtra
• Saggio: ≥99.5% (titration)
• Stato: powder
• Impurezze: Insoluble matter, passes filter test
• Residuo alla calcinazione (900 °C): ≤0.1% (as SO₄)
• Perdita: ≤1.0% loss on drying, 110°C
• pH: 5.0-6.5 (1 M in H₂O)
• Intervallo di pH utile: 6.8-8.2
• pKa (25 °C): 7.5
• Solubilità: H₂O: 1 M, clear, colorless
• Anioni in tracce: chloride (Cl^-): ≤0.05%
• Cationi in tracce: Al: ≤0.0005%; As: ≤0.0001%; Ba: ≤0.0005%; Bi: ≤0.0005%; Ca: ≤0.001%; Cd: ≤0.0005%; Co: ≤0.0005%; Cr: ≤0.0005%; Cu: ≤0.0005%; Fe: ≤0.0005%; K: ≤0.005%; Li: ≤0.0005%; Mg: ≤0.0005%; Mn: ≤0.0005%; Mo: ≤0.0005%; Na: ≤0.005%; Ni: ≤0.0005%; Pb: ≤0.0005%; Sr: ≤0.0005%; Zn: ≤0.0005%
• Assorbimento: ≤0.035 at 280 in H₂O at 1 M; ≤0.045 at 260 in H₂O at 1 M
• applicazioni: diagnostic assay manufacturing
• Stringa SMILE: OCCN1CCN(CC1)CCS(O)(=O)=O
• InChI: 1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14); JKMHFZQWWAIEOD-UHFFFAOYSA-N

Descrizione

Descrizione generale

HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.

Applicazioni

HEPES has been used:

• As a component of homogenisation buffer used in protein sample preparation
• To neutralise PRP (platelet-rich plasma) solution as part of PRP preparation
• As a component in 2X PBS buffer, which is used in phosphate calcium transfection in cell line preparation
• As a supplement in RPMI 1640 medium used to culture human umbilical vein endothelial cells (HUVEC) and in M199 complete medium for ex-vivo treatment of testicular tissue.

Altre note

Easily compare specifications for HEPES products with the HEPES specification table.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 13 - Non Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable
• Dispositivi di protezione individuale: Eyeshields, Gloves, type N95 (US)