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Key Documents

H3662

Sigma-Aldrich

HEPES sodium salt solution

BioReagent, 1M, suitable for cell culture

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About This Item

Formula empirica (notazione di Hill):
C8H17N2NaO4S
Numero CAS:
Peso molecolare:
260.29
Numero MDL:
Codice UNSPSC:
12161700
ID PubChem:
NACRES:
NA.25

Livello qualitativo

Sterilità

sterile-filtered (0.2 μm filtered)

Nome Commerciale

BioReagent

Forma fisica

solution

Concentrazione

0.90-1.10 M

tecniche

cell culture | mammalian: suitable

Impurezze

Bioburden, tested
DNase, RNase. Protease, Nickase, free
endotoxin, tested
<5 ppm Heavy metals (as Pb)

Cationi in tracce

Fe: <5 ppm

Stringa SMILE

[Na+].OCCN1CCN(CC1)CCS([O-])(=O)=O

InChI

1S/C8H18N2O4S.Na/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14;/h11H,1-8H2,(H,12,13,14);/q;+1/p-1
RDZTWEVXRGYCFV-UHFFFAOYSA-M

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Descrizione generale

HEPES is a general-purpose zwitterionic buffer which does not bind magnesium, calcium, manganese(II) or copper(II) ions. HEPES has been classified as Good′s buffer by Dr. Norman Good and his colleagues in 1966. It is zwitterionic at biological pH and is most effective in the pH range of pH 6.8 to 8.2.

Applicazioni

HEPES sodium salt solution is suitable for use in cell culture studies. It may be used in the preparation of buffer solution of HEPES. It may be used as buffer during phosphorylation assays in permeabilized fibroblasts. It may be used to compose a complex dialysis buffer, used in the determination of free thyroxin in serum by radioimmunoassay.

Codice della classe di stoccaggio

10 - Combustible liquids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


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