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U7752

Sigma-Aldrich

Urease

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About This Item

Número CAS:
9002-13-5
Número da licença da enzima:
3.5.1.5 (BRENDA, IUBMB)
Número CE:
232-656-0
Número MDL:
MFCD00070858
Código UNSPSC:
12352204
eCl@ss:
32160410
NACRES:
NA.54

fonte biológica

Canavalia ensiformis

Nível de qualidade

200

forma

solid

peso molecular

hexamer 545 kDa
trimer ~272 kDa

contém

~20% dithiothreitol

adequação

suitable for marker for electrophoresis (non-denaturing PAGE)

temperatura de armazenamento

−20°C

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Descrição geral

Peso da subunidade molecular: aprox. 90.770
Composta por seis subunidades com peso molecular total: aprox. 544.620
Urease is a multi-subunit nickel-dependent metalloenzyme. It is found abundantly in fungi, yeast, plants, and bacteria. It belongs to the superfamily of amidohydrolases and phosphotriesterases. Urease is also found in leguminous seeds.

Aplicação

Urease from Canavalia ensiformis (Jack bean) has been used:
  • as a component of the molecular mass standard in electrophoresis
  • as a component of the standard in size-exclusion chromatography
  • as a reference in native-polyacrylamide gel electrophoresis (PAGE)

Ações bioquímicas/fisiológicas

Urease is an essential enzyme of the nitrogen cycle. It plays a role in degrading urea into ammonia and carbamate. Urease provides a source of nitrogen for growth to various organisms. It also plays a major role in seed germination and seed chemical defense.

Outras notas

Contains approx. 1 mg protein

Pictogramas

Health hazardExclamation mark
GHS08,GHS07

Palavra indicadora

Danger

Frases de perigo

H315,H319,H334,H335

Classificações de perigo

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Órgãos-alvo

Respiratory system

Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 1

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Muhammad Ajmal Rana et al.
PloS one, 16(10), e0258568-e0258568 (2021-10-15)
Urea is the most popular and widely used nitrogenous fertilizer. High soil urease activity rapidly hydrolyses applied urea to ammonia which contributes to soil nitrogen (N) losses and reduces N use efficiency of crop plants. The ammonia losses can be
Anuradha Balasubramanian et al.
Journal of molecular biology, 400(3), 274-283 (2010-05-18)
Urease, a nickel-dependent metalloenzyme, is synthesized by plants, some bacteria, and fungi. It catalyzes the hydrolysis of urea into ammonia and carbon dioxide. Although the amino acid sequences of plant and bacterial ureases are closely related, some biological activities differ
Prevention of infected urinary stones by urease inhibition.
D P Griffith et al.
Investigative urology, 11(3), 228-233 (1973-11-01)
Paweł Kafarski et al.
Journal of advanced research, 13, 101-112 (2018-08-11)
Urease is a nickel-dependent metalloenzyme found in plants, some bacteria, and fungi. Bacterial enzyme is of special importance since it has been demonstrated as a potent virulence factor for some species. Especially it is central to Helicobacter pylori metabolism and
Suchithra Menon et al.
Cell, 156(4), 771-785 (2014-02-18)
mTORC1 promotes cell growth in response to nutrients and growth factors. Insulin activates mTORC1 through the PI3K-Akt pathway, which inhibits the TSC1-TSC2-TBC1D7 complex (the TSC complex) to turn on Rheb, an essential activator of mTORC1. However, the mechanistic basis of how
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