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Documentos Principais

MAK120

Sigma-Aldrich

Urease Activity Assay Kit

sufficient for 100 colorimetric tests

Sinônimo(s):

Urea Amidohydrolase Activity Assay Kit

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About This Item

Código UNSPSC:
12161503
NACRES:
NA.84

uso

sufficient for 100 colorimetric tests

aplicação(ões)

environmental

método de detecção

colorimetric

doença(s) relevante(s)

gastrointestinal diseases

temperatura de armazenamento

2-8°C

Categorias relacionadas

Descrição geral

Urease is an enzyme that catalyzes the hydrolysis of urea into carbon dioxide and ammonia. Urease is found in bacteria, yeast, and certain higher plant species. Urease activity is found in environmental and fecal samples.

Aplicação

Urease Activity Assay Kit has been used to measure urease activity.

Características e benefícios

Compatible with high-throughput handling systems. Can be adapted for use with cuvettes.

Adequação

Suitable for the detection of Urease activity in biological and environmental samples.

Princípio

In this assay, urease catalyzes the hydrolysis of urea resulting in the production of ammonia. The ammonia is determined by the Berthelot method resulting in colorimetric product measure at 670 nm, proportionate to the urease activity present in the sample. One unit of urease is the amount of enzyme that catalyzes the formation of 1.0 μmole ammonia per minute at pH 7.0.

Palavra indicadora

Danger

Frases de perigo

H290,H314,H341,H410

Classificações de perigo

Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Met. Corr. 1 - Muta. 2 - Skin Corr. 1B

Perigos de suplementos

EUH031

Código de classe de armazenamento

8B - Non-combustible corrosive hazardous materials

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Escolha uma das versões mais recentes:

Certificados de análise (COA)

Lot/Batch Number
120CC01A10
120CB11A18
120CB05A27
120CB02A08
120CA06A25

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Urease, Canavalia ensiformis Native urease from Canavalia ensiformis. Catalyzes hydrolysis of urea to carbon dioxide and ammonia. Useful in the determination of urea in body fluids. Note: 1 KU = 1000 units.

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Isolation, partial purification, characterization and inhibition of urease (EC 3.5. 1.5) enzyme from the Cajanus cajan seeds.
Sujoy B and Aggarwal A
Asian Journal of Bio Science, 7(2), 203-209 (2012)
Joudeh B Freij et al.
Infection and immunity, 86(7) (2018-05-02)
The genus Cryptococcus includes several species pathogenic for humans. Until recently, the two major pathogenic species were recognized to be Cryptococcus neoformans and Cryptococcus gattii We compared the interaction of murine macrophages with three C. gattii species complex strains (WM179
Short-term treatment with the urease inhibitor N-(n-butyl) thiophosphoric triamide (NBPT) alters urea assimilation and modulates transcriptional profiles of genes involved in primary and secondary metabolism in maize seedlings.
Zanin L, et al.
Frontiers in Plant Science, 7, 845-845 (2016)
Chelsea M Heveran et al.
Scientific reports, 9(1), 14721-14721 (2019-10-13)
We demonstrate for the first time that the morphology and nanomechanical properties of calcium carbonate (CaCO3) can be tailored by modulating the precipitation kinetics of ureolytic microorganisms through genetic engineering. Many engineering applications employ microorganisms to produce CaCO3. However, control
Ureases I. Functional, catalytic and kinetic properties: A review
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