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Key Documents

AA0100

Sigma-Aldrich

Ammonia Assay Kit

sufficient for 100 assays

Sinônimo(s):

Ammonia Quantitative Test Kit

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About This Item

Código UNSPSC:
12352202
NACRES:
NA.84

uso

sufficient for 100 assays

Nível de qualidade

temperatura de armazenamento

2-8°C

Aplicação

Ammonia concentration was measured in serum and urine using the Sigma Ammonia Assay Kit in a study assessing expression of renal ammonia transporters in response to chromic metabolic acidosis.
This kit is for the quantitative, enzymatic determination of ammonia in food and biological samples. Ammonia reacts with α-ketoglutaric acid (KGA) and reduced nicotinamide adenine dinucleotide phosphate (NADPH) in the presence of L-glutamate dehydrogenase (GlDH) to form L-glutamate and oxidized nicotinamide adenine dinucleotide phosphate (NADP+). The decrease in absorbance at 340 nm, due to the oxidation of NADPH, is proportional to the ammonia concentration. L-Glutamate dehydrogenase reacts specifically with ammonia. The Ammonia Assay Kit may be used to determine ammonia concentrations in the range of 0.2-15 μg/ml.

Somente componentes do kit

Nº do produto
Descrição

  • Ammonia Assay Reagent 10 vial(s)

  • L-Glutamate Dehydrogenase for Ammonia Assay 1 mL/vial

  • Ammonia Standard Solution 1 mL/vial

Pictogramas

Health hazard

Palavra indicadora

Danger

Frases de perigo

Classificações de perigo

Eye Irrit. 2 - Resp. Sens. 1

Código de classe de armazenamento

10 - Combustible liquids

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable


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