P3397

Phosphoglucomutase from rabbit muscle

ammonium sulfate suspension, ≥100 units/mg protein

• Sinônimo(s): PGM, α-D-Glucose-1,6-bisphosphatase, α-D-Glucose-1-phosphate phosphotransferase
• Número CAS: 9001-81-4
• Número da licença da enzima: 5.4.2.2 (BRENDA, IUBMB)
• Número MDL: MFCD00131886
• Código UNSPSC: 12352204
• NACRES: NA.54

Propriedades

• fonte biológica: rabbit muscle
• Nível de qualidade: 300
• descrição: ammonium sulfate suspension, >=100 units/mg protein
• Formulário: ammonium sulfate suspension
• atividade específica: ≥100 units/mg protein
• condição de armazenamento: (Tightly closed)
• técnica(s): activity assay: suitable
• cor: white to light yellow
• atividade externa: lactic dehydrogenase ≤0.5%; phosphoglucose isomerase ≤0.01%; pyruvate kinase ≤0.05%
• Condições de expedição: wet ice
• temperatura de armazenamento: 2-8°C

Descrição

Descrição geral

Research area: Cell Signaling

Phosphoglucomutase (PGM), a conserved enzyme, is abundantly found in animals, plants, and microorganisms. It is distributed in almost all tissues.

Aplicação

Phosphoglycomutase has been used:

• to study glycogenesis and phosphoglycomutase deficiency in humans
• in phosphoglucomutase assays to study metabolic regulation
• for assay A to assay enzymes closely linked to glycogen metabolism in transformed BL21(DE3)C43 cells
• in sucrose synthase (SuSy) andADPglucose pyrophosphorylase (AGPase) assays(3)
• in enzyme activity assay for coupling the reduction of nicotinamide adenine dinucleotide phosphate (NADP⁺)to determine glucose-1-phosphate from sucrose and inorganic phosphate (Pi)

Ações bioquímicas/fisiológicas

Phosphoglucomutase(PGM) enzyme plays a vital role in glycolysis and gluconeogenesis. It is involved in glycogen and trehalose metabolism in insects. PGM participates in the development of plants and some microorganisms. It is essential for proteins, lipids, and nucleic acid metabolism and is crucial for the development of plants because glucose-6-phosphate is a crucial central metabolite. PGM catalyzes the interconversion of glucose-6-phosphate (G-6-P)and glucose-1-phosphate (G-1-P).

Definição da unidade

One unit will convert 1.0 μmole of α-D-glucose 1-phosphate to α-D-glucose 6-phosphate per min at pH 7.4 at 30 °C.

forma física

Crystalline suspension in 3.2 M (NH₄)₂SO₄, pH 6.0 containing 0.01% EDTA

Nota de análise

Protein determined by biuret.

Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): WGK 2
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable
• Equipamento de proteção individual: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)