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P5381

Sigma-Aldrich

Phosphoglucose Isomerase from baker′s yeast (S. cerevisiae)

Type III, ammonium sulfate suspension, ≥400 units/mg protein (biuret)

Sinônimo(s):

D-Glucose-6-phosphate ketol-isomerase, PGI, Phosphosaccharomutase

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About This Item

Número CAS:
9001-41-6
Número da licença da enzima:
5.3.1.9 (BRENDA, IUBMB)
Número CE:
232-603-1
Número MDL:
MFCD00131887
Código UNSPSC:
12352204
NACRES:
NA.54

fonte biológica

bakers yeast

Nível de qualidade

200

tipo

Type III

forma

ammonium sulfate suspension

atividade específica

≥400 units/mg protein (biuret)

peso molecular

tetramer 119,500 Da±600

concentração

≥3 mg/mL protein Biuret

técnica(s)

activity assay: suitable

cor

white

nº de adesão de ID de proteína

NP_009755.1

nº de adesão UniProt

P12709

temperatura de armazenamento

2-8°C

Informações sobre genes

yeast ... PGI1(852495)

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Descrição geral

Research area: Cell Signaling

Phosphoglucose Isomerase ubiquitous multifunctional cytosolic protein. This dimeric protein has a molecular weight of approximately 119,500 ± 600 and is composed of four subunits that are nearly identical.

Aplicação

Phosphoglucose Isomerase from baker′s yeast (S. cerevisiae) has been used:
  • to convert fructose-6-phosphate to glucose-6-phosphate during the enzymatic measurement of serum mannose levels.
  • as one of the enzymes in the series of four enzyme-coupled reactions to measure phosphomannomutase (PMM) activity.
  • to convert fructose to glucose while evaluating fructose content in kudzu roots.

Ações bioquímicas/fisiológicas

Phosphoglucose Isomerase(PGI) is an enzyme crucial for the interconversion of D-glucose 6-phosphate and D-fructose 6-phosphate. PGI is responsible for the second step of glycolysis and is involved in glucogenesis and pentose phosphate pathway (PPP). It is highly conserved in bacteria and eukaryotes. PGI plays a vital role in the survival of A. fumigatus and demonstrates diverse functions that encompass development, biosynthesis of cell wall glucan, and virulence.Phosphoglucose Isomerase functions as an isomerase, neuroleukin, autocrine motility factor, and a differentiation and maturation mediator.

Definição da unidade

One unit will convert 1.0 μmole of D-fructose 6-phosphate to D-glucose 6-phosphate per min at pH 7.4 at 25 °C.

forma física

Crystalline suspension in 2.6 M (NH4)2SO4 containing 0.1 mM magnesium acetate, pH 7.0

Pictogramas

Health hazard
GHS08

Palavra indicadora

Danger

Frases de perigo

H334

Declarações de precaução

P261 - P284 - P501

Classificações de perigo

Resp. Sens. 1

Código de classe de armazenamento

10 - Combustible liquids

Classe de risco de água (WGK)

WGK 1

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves

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S L Lowe et al.
The Journal of biological chemistry, 250(1), 94-99 (1975-01-10)
Bakers' yeast phosphoglucose isomerase was studied by both chemical and physical methods to determine its submit structure. Gel filtration in 6 M guanidine HCl as well as acrylamide gel electrophoresis of sodium dodecyl sulfatedentured phosphoglucose isomerase showed two speices corresponding
Kudzu [Pueraria montana (Lour.) Merr. Variety lobata: A new source of carbohydrate for bioethanol production
Sage RF, et al.
Biomass and Bioenergy, 33, 57-61 (2009)
J Pozueta-Romero et al.
FEBS letters, 291(2), 233-237 (1991-10-21)
The standardized enzyme coupling method for assaying sucrose synthase activities in the direction of sucrose cleavage was reexamined using enzyme preparations from cultured cells of sycamore (Acer pseudoplatanus L.) and spinach leaves (Spinacea oleracea). Both ATP and Tris, commonly utilized
B K Campbell et al.
Reproduction (Cambridge, England), 140(5), 721-732 (2010-08-19)
Glucose is a critical metabolic fuel in most mammals although many foodstuffs also contain high levels of the monosaccharides, galactose and fructose. The aims of this work were to determine the insulin response to challenges of these sugars (experiment 1)
Johannes Yayo et al.
Applied and environmental microbiology, 87(9) (2021-02-21)
The native ability of Clostridium thermocellum to efficiently solubilize cellulose makes it an interesting platform for sustainable biofuel production through consolidated bioprocessing. Together with other improvements, industrial implementation of C. thermocellum, as well as fundamental studies into its metabolism, would
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