H6380

Hexokinase from Saccharomyces cerevisiae

lyophilized powder, ≥350 units/mg protein, Protein ≥10 % by biuret

• Sinônimo(s): ATP:D-Hexose-6-phosphotransferase, Hexokinase from yeast
• Número CAS: 9001-51-8
• Número da licença da enzima: 2.7.1.1 (BRENDA, IUBMB)
• Número CE: 232-611-5
• Número MDL: MFCD00131321
• Código UNSPSC: 12352204
• eCl@ss: 32160410
• NACRES: NA.54

Propriedades

• fonte biológica: Saccharomyces cerevisiae
• Nível de qualidade: 200
• forma: lyophilized powder
• atividade específica: ≥350 units/mg protein
• peso molecular: dimer 110 kDa
• composição: Protein, ≥10% biuret
• fabricante/nome comercial: Sigma-Aldrich
• técnica(s): activity assay: suitable
• cor: white
• pH: 6
• nº de adesão UniProt: P04806; P04807
• aplicação(ões): life science and biopharma
• atividade externa: L-Glutamic dehydrogenase ≤0.05%; Creatine phosphokinase, myokinase, 6-phosphogluconic dehydrogenase and alcohol dehydrogenase ≤0.001%; Glucose-6-phosphate dehydrogenase ≤0.005%; Glutathione reductase ≤0.005%; Phosphoglucose isomerase ≤0.002%
• temperatura de armazenamento: 2-8°C
• Informações sobre genes: bakers yeast ... HXK1(850614) , HXK2(852639)

Descrição

Descrição geral

Research area: Cell signalling. Hexokinase (HK) from yeast is a 486 amino-acid containing enzyme with a α/β fold. It comprises a large and small domain separated by an active site cleft. Conserved glycine residues are essential for ATP and glucose binding. The two isoforms are HKI and HKII encoded by HXK1 and HXK2 gene, respectively.

Aplicação

Hexokinase from Saccharomyces cerevisiae has been used:

• to investigate its amyloid fibril forming tendency using biophysical methods
• in tracking ATP depletion in peroxisomes and reticulocyte lysates in import assays
• in the synthesis of Mg-ADP-actin from Mg-ATP actin monomers

Ações bioquímicas/fisiológicas

Catalyzes the phosphorylation of D-hexose sugars at the C6 position utilizing ATP as a phosphate source.

The rate of phosphorylation varies with different hexoses (pH 7.5, 30 °C).
D-fructose K_M: 0.33 mM
D-glucose K_M: 0.12 mM
D-mannose K_M: 0.05 mM

Yeast hexokinase exists as two similar isoforms, PI and PII (A and B), with isoelectric points of 5.25 and 4, respectively.

Molecular Weight: ~ 54 kDa (monomer)
~110 kDa (dimer)
Optimal pH: 7.5 to 9.0
Extinction Coefficient: E^1% = 8.85 (PI) and 9.47 (PII) at 280 nm

Activators: Hexokinase requires Mg²⁺ ions (K_M = 2.6 mM) for activity. Hexokinase is activated by catecholamines and related compounds.

Inhibitors: sorbose-1-phosphate, polyphosphates, 6-deoxy-6-fluoroglucose, 2-C-hydroxy-methylglucose, xylose, lyxose, and thiol reactive compounds (Hg²⁺ and 4-chloromercuribenzoate)

Hexokinase II is a crucial enzyme in glucose metabolism. Deletion of gene in S. cerevisiae in the presence of high glucose displays oxidative growth and results in the production of high biomass as well as shortened diauxic shift. Hexokinase II is also involved in glucose repression.

Definição da unidade

One unit will phosphorylate 1.0 μmole of D-glucose per min at pH 7.6 at 25 °C, unless otherwise indicated below.

forma física

Lyophilized powder containing phosphate/citrate pH approx. 7.0

Outras notas

Predominantly the PII isoform, produced from an overexpressing yeast strain.

Informações de segurança

• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 3
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable
• Equipamento de proteção individual: Eyeshields, Gloves, type N95 (US)