PGI-RO

Phosphoglucose Isomerase (PGI)

from yeast

• Sinônimo(s): glucose-6-phosphate isomerase
• Número da licença da enzima: 5.3.1.9 (BRENDA, IUBMB)
• Código UNSPSC: 12352200

Propriedades

• fonte biológica: yeast
• Nível de qualidade: 100
• forma: suspension
• atividade específica: ~350 units/mg protein (at 25 °C with fructose-6-P as the substrate.)
• embalagem: pkg of 1 mL (10127396001 [2 mg]); pkg of 1 mL (10128139001 [10 mg])
• fabricante/nome comercial: Roche
• pH ideal: 7.0-7.6
• temperatura de armazenamento: 2-8°C

Descrição

Descrição geral

Phosphoglucose Isomerase is an enzyme which possesses diagnostic potential when fractionated by cellulose acetate electrophoresis. This cytosolic enzyme plays an important role during glycolysis and glucogenesis. It acts as an tumor-secreted cytokine and autocrine motility factor. It is used as neurotrophic factor, maturation factor and as serine proteinase inhibitor.

Especificidade

Phosphoglucose isomerase (PGI) is specific for G6P (K_m = 0.7 mM) and fructose-6-phosphate (F6P).

Aplicação

Isomerization of ketoses to aldoses.

Qualidade

Contaminants: <0.01% F6P-K, GR, 6-PGDH and PGluM, each, <0.2% β-fructosidase

forma física

Suspension in 3.2 M ammonium sulfate solution, pH approximately 6

Nota de preparo

Storage conditions (working solution): A solution of PGI in 20 mM Tris, pH 8.1, containing 0.2 mg BSA/ml, is stable approx. 1 week at 2 to 8 °C.

Outras notas

For life science research only. Not for use in diagnostic procedures.

Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): No data available
• Ponto de fulgor (°C): No data available