LMDH-RO

L-Malate Dehydrogenase (L-MDH)

from pig heart (mitochondrial)

• Número da licença da enzima: 1.1.1.37 (BRENDA, IUBMB)
• Código UNSPSC: 12352202

Propriedades

• fonte biológica: Porcine heart (mitochondrial)
• Nível de qualidade: 100
• Formulário: solution; suspension
• atividade específica: ~1200 units/mg protein (At 25 °C with oxaloacetate as the substrate.)
• embalagem: pkg of 1 mL (10127248001 solution); pkg of 1 mL (10127256001 suspension); pkg of 5 mL (10127914001suspension)
• fabricante/nome comercial: Roche
• técnica(s): activity assay: suitable
• cor: white
• pH ideal: 7.4-7.5(reduction of oxaloacetate); 9.2-9.5(oxidation of malate)
• solubilidade: water: miscible
• adequação: suitable for Western blot
• nº de adesão NCBI: NM_001244153.1
• nº de adesão UniProt: P00346
• aplicação(ões): life science and biopharma
• atividade externa: Fumarase <0.01%; GIDH 0.001%; GOT 0.001%; GPT 0.00185%; LDH <0.01%; NADH oxidase <0.001%
• Condições de expedição: wet ice
• temperatura de armazenamento: 2-8°C
• Informações sobre genes: Porcine ... MDH2(397039)

Descrição

Descrição geral

L-malate:NAD⁺ oxidoreductase
The L-malate dehydrogenase enzyme is a nuclear gene product that is synthesized with a 24-residue amino-terminal signal peptide. This peptide is proteolytically cleaved during the translocation of the enzyme to the mitochondrial matrix.

Aplicação

The enzyme L-malate dehydrogenase from pig heart has been used to measure PEPCK (phosphoenolpyruvate carboxykinase) activity. The oxaloacetate, produced by PEPCK, is reduced to malate via the oxidation of NADH, which in turn is measured at 340 nm using a spectrophotometer. The enzyme has also been used to measure oxaloacetate by measuring the reduction in NADH spectroscopically at 340 nm.

Ações bioquímicas/fisiológicas

The enzyme L-malate dehydrogenase from pig heart catalyzes the oxidation of L-malate to oxaloacetate. The enzyme is an NAD-dependent mitochondrial dehydrogenase that functions in the tricarboxylic acid cycle. It is a component of the malate-aspartate shuttle that transports reducing equivalents across the inner mitochondrial membrane in the form of malate.

Qualidade

Contaminants: <0.002% GOT, <0.01% fumarase and LDH, each luM each, <0.02% HK, <0.002% PGI

forma física

Solution in 50% glycerol (v/v), pH approximately 7

Suspension in 3.2 M ammonium sulfate solution, pH approximately 6

Nota de preparo

Activator: – phosphate
– arsenate
– Mg²⁺
– Zn²⁺

Outras notas

For life science research only. Not for use in diagnostic procedures.

Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): No data available
• Ponto de fulgor (°C): No data available