F1757

Fumarase from porcine heart

ammonium sulfate suspension, ≥300 units/mg protein (biuret)

• Sinônimo(s): L-Malate hydrolyase, Fumarate hydratase
• Número CAS: 9032-88-6
• Número da licença da enzima: 4.2.1.2 (BRENDA, IUBMB)
• Número CE: 232-887-7
• Número MDL: MFCD00131111
• Código UNSPSC: 12352204
• NACRES: NA.54

Propriedades

• Formulário: ammonium sulfate suspension
• Nível de qualidade: 200
• atividade específica: ≥300 units/mg protein (biuret)
• atividade externa: malic dehydrogenase ≤0.1%
• temperatura de armazenamento: 2-8°C

Descrição

Descrição geral

Fumarase (FH in human) is a well-known tricarboxylic-acid-cycle enzyme found in both the cytoplasm and mitochondria of all eukaryotes.

Fumarase is mapped to human chromosome 1q43. Fumarase comprises of three domains D1, D2 and D3 and belongs to class II fumarase family.

Aplicação

Fumarase from porcine heart has been used:

• as a positive control in isocitrate dehydrogenase and α-ketoglutarate dehydrogenase assay
• for immobilized enzyme based stability assay
• in fumarate hydratase assays in human lung carcinoma cells (A549)

Fumarase is used as a protein calibration standard in the purification of intact DNA polymerase a/primase from mouse cells.

Ações bioquímicas/fisiológicas

Fumarase catalyzes the reversible hydration of fumarate to malate. In its mitochondrial form, fumarate is involved in the Krebs Cycle, while the cytosolic form is involved in amino acid metabolism.

Fumarase is essential for regulation of cellular metabolism. Fumarase elicits protective function from double-strand breaks (DSBs) induced by infrared radiation. Loss of function mutations in the fumarase gene is implicated in hereditary leiomyomatosis and renal cell cancer (HLRCC)-syndrome.

Definição da unidade

One unit will convert 1.0 μmole of L-malate to fumarate per min at pH 7.6 at 25 °C.

forma física

Suspension in 3.2 M (NH₄)₂SO₄, 0.05 M KH₂PO₄, pH 7.5, 0.014 M 2-mercaptoethanol

Nota de preparo

Isolated by the method of Beeckmans, S., and Kanarek, L., Eur. J. Biochem., 78, 437 (1977). Affinity purified.

Informações de segurança

• Pictogramas: GHS07
• Palavra indicadora: Warning
• Frases de perigo: H317
• Declarações de precaução: P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
• Classificações de perigo: Skin Sens. 1
• Código de classe de armazenamento: 10 - Combustible liquids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable