H2625

Hemoglobin from bovine blood

suitable for protease substrate, substrate powder

• Sinônimo(s): Bovine hemoglobin, Hb, Methemoglobin
• Número CAS: 9008-02-0
• Número MDL: MFCD00131282
• Código UNSPSC: 12352202
• eCl@ss: 42030116
• NACRES: NA.61

Propriedades

• fonte biológica: bovine blood
• Nível de qualidade: 300
• forma: substrate powder
• peso molecular: M_r ~64500
• técnica(s): activity assay: suitable
• solubilidade: H₂O: soluble 20 mg/mL
• adequação: suitable for protease substrate
• nº de adesão UniProt: P01966; P02070; P02081; P06642; P06643
• temperatura de armazenamento: 2-8°C
• Informações sobre genes: cow ... HBA1(281221) , HBB(280813) , HBE2(513240) , HBE4(513108) , HBG(511735) , alpha globin(512439)

Descrição

Descrição geral

Hemoglobin is the major component of red blood cells and is responsible for their red color. Its normal concentration in erythrocytes is 34%. Hemoglobin is a globular protein with α and β chains with every 141 and 146 amino acids respectively. It exists as a tetramer with each monomer having heterocyclic porphyrin ring with iron constituting the heme.

Hemoglobin is the major component of red blood cells, and is responsible for their red color. Its normal concentration in erythrocytes is 34%. Hemoglobin is the most important respiratory protein of vertebrates by virtue of its ability to transport oxygen from the lungs to body tissues, and to facilitate the return transport of carbon dioxide.

Aplicação

Hemoglobin from bovine blood has been used as a substrate in cathepsin D activity assay. It has also been used to measure the activity of acid proteases (pepsin-like) in the stomach extract.

The solubility of α-elastin has been applied to construction of elastin-mimetic biomaterials.

Ações bioquímicas/fisiológicas

Bovine hemoglobin is used in the production of hemoglobin-vesicles (HbV). It has high thermal stability and high oxygen affinity when compared to human hemoglobin. Bovine hemoglobin interacts with synthetic and azo dyes. Polymerized forms of bovine hemoglobin are used to treat autoimmune hemolytic anemia.

Oxygen transporter. The Fe²⁺/Fe³⁺ balance is a physiological indicator of blood oxygenation; deoxygenated hemoglobin accessorizes a feedback loop by reducing nitrite to NO, a vasodilator which enhances blood flow to oxygen-deprived tissues.

Atenção

Since native hemoglobin is readily oxidized in air, these preparations may be predominantly methemoglobin.

Nota de preparo

Prepared from washed, lysed and dialyzed erythrocytes.

Informações de segurança

• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 3
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable
• Equipamento de proteção individual: Eyeshields, Gloves, type N95 (US)