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P8849

Sigma-Aldrich

Protease Inhibitor Cocktail

DMSO solution, for the inhibition of serine, cysteine, aspartic, aminopeptidases and thermolysin-like activities, for use in purification of Histidine-tagged proteins, DMSO solution

Sinônimo(s):

Protease inhibitor solution

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About This Item

Número MDL:
Código UNSPSC:
12352200
NACRES:
NA.77

product name

Coquetel Inibidor de Protease, for use in purification of Histidine-tagged proteins, DMSO solution

fonte biológica

synthetic

Nível de qualidade

forma

DMSO solution

temperatura de armazenamento

−20°C

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Descrição geral

DMSO solution designed for use in the purification of histidine-tagged proteins.

The cocktail contains a mixture of inhibitors that specifically target serine, cysteine, aspartic, and thermolysin-like proteases, and aminopeptidases.

Especificidade

Inhibits serine, cysteine, aspartic, and thermolysin-like proteases, and aminopeptidases.

Aplicação

The cocktail has been optimized and tested for isolation of histidine-tagged proteins, with chelating agents omitted for compatibility with IMAC applications.

It is recommended for inhibition of protease activity in 100 mL of cell lysate from 20 g of Escherichia coli or 10 g of baculovirus-infected Spodoptera frugiperda pupal ovary cells.

Características e benefícios

Specifically formulated for histidine-tagged protein purification.

Targets multiple types of proteases, ensuring comprehensive inhibition of protease activity.

Compatibility with IMAC applications due to omission of chelating agents.

Supplied in convenient packaging options.

Componentes

AEBSF
Bestatin
E-64
Pepstatin A
Phosphoramidon

Quantidade

One mL is recommended for the inhibition of proteases extracted from 20 g of Escherichia coli or 10 g of baculovirus-infected Spodoptera frugiperda pupal ovary cells in a total volume of 100 ml.

This protease inhibitor cocktail has been optimized and tested for histidine-tagged proteins

Nota de preparo

This product is supplied as a clear solution in DMSO. One mL of solution is recommended for inhibition of protease activity in 100 mL of cell lysate from 20 g of E. coli cells or 10 g of baculovirus-infected cells.

Código de classe de armazenamento

10 - Combustible liquids

Classe de risco de água (WGK)

WGK 1

Ponto de fulgor (°F)

185.0 °F - closed cup

Ponto de fulgor (°C)

85 °C - closed cup


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