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P8465

Sigma-Aldrich

Protease Inhibitor Cocktail

lyophilized powder, for the inhibition of serine, cysteine, aspartic, metalloproteases and aminopeptidases, for use with bacterial cell extracts, lyophilized powder

Sinônimo(s):

Protease inhibitor

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About This Item

Código UNSPSC:
12352200
NACRES:
NA.77

Nome do produto

Coquetel inibidor de protease em pó, for use with bacterial cell extracts, lyophilized powder

Nível de qualidade

Formulário

lyophilized powder

temperatura de armazenamento

−20°C

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Descrição geral

The Protease Inhibitor Cocktail is a lyophilized powder for use in the inhibition of proteases present in bacterial cell extracts.

The product contains individual components that target serine, cysteine, aspartic, and metalloproteases, as well as aminopeptidases.

Aplicação

The protease inhibitor cocktail improves the yields of intact proteins by adding inhibitors to enzymes that modify proteins present in cell extracts. This product has been optimized and tested for bacterial cell use, with broad specificity against serine, cysteine, and aspartic proteases, metalloproteases, and aminopeptidases.

Ações bioquímicas/fisiológicas

This mixture contains individual components, including AEBSF at 23 mM, EDTA at 100 mM, Bestatin at 2 mM, Pepstatin A at 0.3 mM, and E-64 at 0.3 mM. AEBSF acts to inhibit serine proteases, including trypsin, chymotrypsin, and plasmin amongst others. Bestatin inhibits aminpeptidases. E-64 acts against cystein proteases. Pepstatin A inhibits acid proteases. EDTA is an inhibitor of metalloproteases.

Características e benefícios

Broad specificity against serine, cysteine, aspartic, and metalloproteases, as well as aminopeptidases.

Contains individual components, including AEBSF, EDTA, Bestatin, Pepstatin A, and E-64, each targeting specific types of proteases.

Lyophilized powder is stable for at least 2 years when stored unopened at -20°C.

Supplied with a vial of DMSO for preparation of a cocktail solution.

One mL of the cocktail solution is recommended for the inhibition of protease activity found in 20 mL of cell lysate from 4g of E. coli cells.

Atenção

The lyophilized powder is stable for at least 2 years when stored unopened at -20°C. It is also supplied with a vial of DMSO. A prepared solution in DMSO and water will remain clear and colorless for approximately 24 hours at 4°C, before the inhibitors will precipitate out.

Nota de preparo

A cocktail solution may be prepared by adding 1 mL of DMSO and 4 mL of deionized water to the 5 mL size or 5 mL of DMSO and 20 mL of deionized water to the 25 mL size. One mL of the cocktail solution is recommended for the inhibition of the protease activity found in 20 mL of cell lysate from 4g of E. coli cells.

Palavra indicadora

Danger

Frases de perigo

Classificações de perigo

Acute Tox. 4 Inhalation - Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1A - STOT RE 2 Inhalation

Órgãos-alvo

Respiratory Tract

Código de classe de armazenamento

8A - Combustible corrosive hazardous materials

Classe de risco de água (WGK)

WGK 3

Equipamento de proteção individual

Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges


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