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Merck
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Key Documents

539131

Sigma-Aldrich

Protease Inhibitor Cocktail I

lyophilized, for the inhibition of aspartic, cysteine, serine, metalloproteases and aminopeptidases

Sinônimo(s):

Protease Inhibitor Mix, Protease inhibitor

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About This Item

Código UNSPSC:
12352200
NACRES:
NA.28

product name

Protease Inhibitor Cocktail Set I, A cocktail of five protease inhibitors that will inhibit a broad range of proteases and esterases. Supplied with a data sheet.

forma

lyophilized

fabricante/nome comercial

Calbiochem®

condição de armazenamento

OK to freeze
desiccated (hygroscopic)

Condições de expedição

wet ice

temperatura de armazenamento

−20°C

Descrição geral

Protease Inhibitor Cocktail Set I A is specially formulated containing five protease inhibitors that will inhibit a broad range of proteases and esterases.

Especificidade

Inhibits a broad range of proteases and esterases.

Aplicação

Protease Inhibitor Cocktail Set I – Calbiochem has been used in lysis buffer to lyse cells and perform western blotting.
Protease Inhibitor Cocktail Set I have been used as a supplement in:
  • cold radioimmunoprecipitation assay (RIPA) buffer to prepare cell lysates for western blotting
  • RIPA lysis buffer to extract total protein from rat liver tissues and AML12 cells
  • sucrose solution to homogenize mice cortices for myelin isolation

Ações bioquímicas/fisiológicas

Protease inhibitor cocktails and specific protease inhibitors are essential tools utilized by scientists in various proteomic studies. These studies encompass protein expression, characterization, biomarker discovery, mapping post-translational modifications, protein expression profiling, and quantitative protein measurement. The application of a protease inhibitor cocktail or protease inhibitors guarantees the preservation of protein integrity, facilitating downstream analysis and characterization.

Características e benefícios

  • Protease Inhibitor Cocktails offer protection of target protein against degradation in crude extracts
  • Ready for reconstitution

Advertência

Toxicity: Harmful (C)

Reconstituição

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 1 month at -20°C.
Reconstitute each vial with 1 ml dH2O to yield a 100X stock solution.

Outras notas

The presence of EDTA may interfere with the purification of His•Tag fusion proteins if a cell extract is applied directly to IMAC resins.

Informações legais

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Palavra indicadora

Danger

Frases de perigo

Classificações de perigo

Acute Tox. 4 Inhalation - Eye Dam. 1 - Skin Corr. 1A - STOT RE 2 Inhalation

Órgãos-alvo

Respiratory Tract

Código de classe de armazenamento

8B - Non-combustible corrosive hazardous materials

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable


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