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P7634

Sigma-Aldrich

3-Phosphoglyceric Phosphokinase from baker′s yeast (S. cerevisiae)

ammonium sulfate suspension, ≥500 units/mg protein

Sinônimo(s):

PGK, Phosphoglycerate kinase, 3-Phosphoglycerate kinase, ATP:3-Phospho-D-glycerate 1-phosphotransferase

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About This Item

Número CAS:
9001-83-6
Número da licença da enzima:
2.7.2.3 (BRENDA, IUBMB)
Número CE:
232-636-1
Número MDL:
MFCD00130301
Código UNSPSC:
12352204
eCl@ss:
32160410
NACRES:
NA.54

fonte biológica

bakers yeast

forma

ammonium sulfate suspension

atividade específica

≥500 units/mg protein

condição de armazenamento

(Tightly closed)

concentração

1.0-10.0 mg/mL

atividade externa

Glyceraldehyde-3-phosphate dehydrogenase ≤0.1%

Condições de expedição

wet ice

temperatura de armazenamento

2-8°C

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Descrição geral

Research area: Cell Signaling

Phosphoglycerate kinase (PGK), a glycolytic enzyme, is isolated from a broad variety of organisms. This typical hinge-bending monomeric enzyme is well-conserved among the three domains of life. The enzyme is made up of a single folded polypeptide chain that divides into two nearly identical domains, each linked by two -helices (-helices 7 and 14) and separated by a deep cleft. This arrangement gives the enzyme its distinctive bilobed structure.

Aplicação

3-Phosphoglyceric Phosphokinase from baker′s yeast (S. cerevisiae) has been used:
  • to study low molecular weight GTP-binding proteins and mechanisms of inhibition of glyceraldehyde-3-phosphate dehydrogenase
  • in the coupled assay to measure the backward activity of purified rabbit skeletal muscle nicotinamide adenine dinucleotide (NAD+)-dependent glyceraldehyde3-phosphate dehydrogenase (GAPDH)
  • in the assay of glyceraldehyde-3-phosphate dehydrogenase

Ações bioquímicas/fisiológicas

3-Phosphoglyceric phosphokinase catalyzes the reversible transfer of a phosphate group from 1,3-diphosphoglycerate to ADP to generate ATP and 3-phosphoglycerate. 3-Phosphoglycerate phosphokinase activity is essential for glycolysis and gluconeogenesis. Phosphoglyceratekinase (PGK) plays a vital role in the glycolytic pathway by catalyzing one of the two ATP-producing reactions. It converts 1,3-bisphosphoglycerate (1,3BPGA) to 3-phosphoglycerate (3PGA). Additionally, it takes part in the process of gluconeogenesis by catalyzing the opposite reaction to create 1,3BPGA and adenosine diphosphate (ADP). Due to its participation in numerous processes other than energy metabolisms, such as pathogenesis, interaction with nucleic acids, tumorigenesis progression, cell death, and viral replication, PGK is also known as a moonlighting protein.

Definição da unidade

One unit will convert 1.0 μmole of 1,3-diphosphoglycerate to 3-phosphoglycerate per min at pH 6.9 at 25 °C.

forma física

Crystalline suspension in 3.0 M (NH4)2SO4 and 0.04 M tetrasodium pyrophosphate solution, pH 8.0

Nota de análise

Protein determined by TCA Biuret.

Pictogramas

Exclamation mark
GHS07

Palavra indicadora

Warning

Frases de perigo

H319

Classificações de perigo

Eye Irrit. 2

Código de classe de armazenamento

12 - Non Combustible Liquids

Classe de risco de água (WGK)

WGK 2

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves, type N95 (US)

Certificados de análise (COA)

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J P Doucet et al.
The Journal of biological chemistry, 266(26), 17613-17620 (1991-09-15)
The presence of low molecular weight GTP-binding proteins was investigated in subcellular fractions from skeletal muscle. Skeletal muscle homogenate, transverse tubules, triads, sarcoplasmic reticulum membranes, and cytosol fractions were separated in sodium dodecyl sulfate-gel electrophoresis and blotted onto nitrocellulose. The
I Pérez-Dorado et al.
Molecular oral microbiology, 27(4), 221-245 (2012-07-05)
Surface-exposed proteins of pathogenic bacteria are considered as potential virulence factors through their direct contribution to host-pathogen interactions. Four families of surface proteins decorate the cell surface of the human pathogen Streptococcus pneumoniae. Besides lipoproteins and LPXTG proteins, also present
Katarzyna A Broniowska et al.
American journal of physiology. Heart and circulatory physiology, 299(4), H1212-H1219 (2010-08-03)
S-nitrosothiols are nitric oxide (NO)-derived molecules found in biological systems. They have been variously discussed as both NO reservoirs and as major actors in NO-dependent, but cGMP-independent, signal transduction. Although S-nitrosation of specific cysteine residues has been suggested to represent
Angelika B Riemer et al.
Experimental dermatology, 21(8), 625-629 (2012-07-11)
Based on the exquisite sensitivity, reproducibility and wide dynamic range of quantitative reverse-transcription real-time polymerase chain reaction (qRT-PCR), it is currently the gold standard for gene expression studies. Target gene expression is calculated relative to a stably expressed reference gene.
Kayla Baretta et al.
Acta crystallographica. Section F, Structural biology and crystallization communications, 68(Pt 7), 790-792 (2012-07-04)
Acinetobacter baumannii is a common multidrug-resistant clinical pathogen that is often found in hospitals. The A. baumannii phosphoglycerate kinase (AbPGK) is involved in the key energy-producing pathway of glycolysis and presents a potential target for antibiotic development. AbPGK has been
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