G2267

Glyceraldehyde-3-phosphate Dehydrogenase from rabbit muscle

lyophilized powder, ≥75 units/mg protein

• Sinônimo(s): D-Glyceraldehyde 3-phosphate:NAD⁺ oxidoreductase (phosphorylating), GAPDH
• Número CAS: 9001-50-7
• Número da licença da enzima: 1.2.1.12 (BRENDA, IUBMB)
• Número CE: 232-609-4
• Número MDL: MFCD00131203
• Código UNSPSC: 12352204
• NACRES: NA.54

Propriedades

• fonte biológica: rabbit muscle
• Nível de qualidade: 200
• Formulário: lyophilized powder
• atividade específica: ≥75 units/mg protein
• composição: Protein, ≥80% biuret
• atividade externa: 3-Phosphoglyceric phosphokinase ≤0.05%; lactic dehydrogenase, myokinase, and pyruvate kinase ≤0.01%; triosephosphate isomerase ≤0.1%
• Condições de expedição: wet ice
• temperatura de armazenamento: −20°C

Descrição

Descrição geral

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a multifunctional protein. It is basically called as a glycolytic enzyme.

Aplicação

Glyceraldehyde-3-phosphate Dehydrogenase from rabbit muscle has been used:

• for measurements of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) activity assay
• to generate a linear standard curve to analyse GAPDH activity in the experimental samples collected from mice
• in anti-aggregation assays
• in purified GAPDH studies

Ações bioquímicas/fisiológicas

Glyceraldehyde-3-phosphate dehydrogenase catalyzes the conversion of glyceraldehyde-3-phosphate into D-glycerate-1,3-bisphosphate as part of the glycolysis pathway.

Glyceraldehyde-3-phosphate dehydrogenase catalyzes the conversion of glyceraldehyde-3-phosphate to 1,3-bisphosphoglycerate as part of glycolysis. It has also been shown to have roles in initiation of apoptosis, transcription activation and the shuttling of ER to Golgi vesicles<<<18,New>>>.

Definição da unidade

One unit will reduce 1.0 μmole of 3-phosphoglycerate to D-glyceraldehyde 3-phosphate per min in a coupled system with 3-phosphoglyceric phosphokinase at pH 7.6 at 25 °C.

forma física

Lyophilized powder containing citrate buffer salts

Informações de segurança

• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 3
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable
• Equipamento de proteção individual: Eyeshields, Gloves, type N95 (US)