M4159

Methylene Blue hydrate

suitable for nucleic acid staining, BioReagent

• Sinônimo(s): 3,7-Bis(dimethylamino)phenothiazin-5-ium chloride xhydrate, Methylene Blue monohydrate, Methylene blue, Nucleic acid staining reagent
• Fórmula empírica (Notação de Hill): C₁₆H₁₈ClN₃S · xH₂O
• Número CAS: 122965-43-9
• Peso molecular: 319.85 (anhydrous basis)
• Beilstein: 3599847
• Número CE: 200-515-2
• Número MDL: MFCD00150006
• Código UNSPSC: 12171500
• ID de substância PubChem: 57654386
• NACRES: NA.52

Propriedades

• grau: for molecular biology
• Nível de qualidade: 100
• linha de produto: BioReagent
• Formulário: powder
• técnica(s): nucleic acid detection: suitable
• solubilidade: water: 1 mg/mL (dark blue)
• adequação: suitable for nucleic acid staining
• temperatura de armazenamento: room temp
• cadeia de caracteres SMILES: O.[Cl-].CN(C)c1ccc2N=C3C=C\C(C=C3Sc2c1)=[N+](/C)C
• InChI: 1S/C16H18N3S.ClH.H2O/c1-18(2)11-5-7-13-15(9-11)20-16-10-12(19(3)4)6-8-14(16)17-13;;/h5-10H,1-4H3;1H;1H2/q+1;;/p-1
• chave InChI: WQVSELLRAGBDLX-UHFFFAOYSA-M

Descrição

Descrição geral

Methylene blue is a heterocyclic aromatic chemical, appearing as a dark green solid powder, changing to blue in aqueous solution. It is used as a stain in many different histology procedures, as well as in molecular biology laboratories.

Aplicação

Methylene Blue hydrate is suitable for use as a nucleic acid stain for agarose and polyacrylamide gel electrophoresis (PAGE). It was used to stain 4T1 tumor cells to study metastasis of tumor cells using clonogenic assay. Methylene blue hydrate crystals were encapsulated in apoferritin nanocages for photodynamic therapy against tumor cells.

Características e benefícios

• Less hazardous than staining with ethidium bromide
• Viewable in visible light
• Fast and easy to use

Informações de segurança

• Pictogramas: GHS07
• Palavra indicadora: Warning
• Frases de perigo: H302
• Classificações de perigo: Acute Tox. 4 Oral
• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 3
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable
• Equipamento de proteção individual: dust mask type N95 (US), Eyeshields, Gloves