199664

Sudan Black B

certified by the Biological Stain Commission

• Sinônimo(s): Ceres black BN, Fat Black HB, Solvent Black 3
• Fórmula empírica (Notação de Hill): C₂₉H₂₄N₆
• Número CAS: 4197-25-5
• Peso molecular: 456.54
• Número do índice de cores: 26150
• Beilstein: 723248
• Número CE: 224-087-1
• Número MDL: MFCD00006919
• Código UNSPSC: 12171500
• ID de substância PubChem: 24851932
• NACRES: NA.47

Propriedades

• grau: certified by the Biological Stain Commission
• Nível de qualidade: 200
• forma: powder
• cor: dark brown to very dark brown
• pf: 120-124 °C (lit.)
• λmax: 415 nm (2nd); 598 nm
• ε (coeficiente de extinção): ≥20000 at 596-603 nm in ethanol
• aplicação(ões): diagnostic assay manufacturing; hematology; histology
• temperatura de armazenamento: room temp
• cadeia de caracteres SMILES: CC1(C)Nc2cccc3c(ccc(N1)c23)\N=N\c4ccc(\N=N\c5ccccc5)c6ccccc46
• InChI: 1S/C29H24N6/c1-29(2)30-26-14-8-13-22-25(17-18-27(31-29)28(22)26)35-34-24-16-15-23(20-11-6-7-12-21(20)24)33-32-19-9-4-3-5-10-19/h3-18,30-31H,1-2H3/b33-32+,35-34+
• chave InChI: YCUVUDODLRLVIC-VPHDGDOJSA-N

Descrição

Aplicação

Sudan Black B has been used in brain BLAQ (Block Lipids and Aldehyde Quench) technique and is used in lipid detection.

Ações bioquímicas/fisiológicas

Sudan Black B has been used to stain phospholipids and intracellular lipids. Staining pattern is almost similar to myeloperoxidase reaction, with positive staining of granulocytic cells, eosinophils and weak monocytic staining.It doesn′t stain lymphocytes. Sudan Black B can be used to stain older blood or bone marrow smears, but the stain slightly fades over time. It is considered to be a most sensitive and specific reagent for lipid staining. Sudan Black B stains phospholipids gray and neutral fats blue-black.

Adequação

Certified as a fat staining using the Churukian variant of the Lillie-Ashburn supersaturated isopropanol method. Sudan III is substituted for the oil red O in the procedure. Burdon′s stain for bacterial lipid.

Informações de segurança

• Pictogramas: GHS08
• Palavra indicadora: Warning
• Frases de perigo: H341
• Declarações de precaução: P201 - P202 - P280 - P308 + P313 - P405 - P501
• Classificações de perigo: Muta. 2
• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 3
• Equipamento de proteção individual: Eyeshields, Gloves, type N95 (US)