28514

Methylene Blue hydrate

≥95% (calc. to the dried substance)

• Sinônimo(s): Basic Blue 9 monohydrate ; Methylene Blue (hydrate)
• Fórmula empírica (Notação de Hill): C₁₆H₁₈ClN₃S · xH₂O
• Número CAS: 122965-43-9
• Peso molecular: 319.85 (anhydrous basis)
• Beilstein: 3599847
• Número CE: 200-515-2
• Número MDL: MFCD00150006
• Código UNSPSC: 12171500
• ID de substância PubChem: 329753217
• NACRES: MA.02

Propriedades

• Nível de qualidade: 200
• Ensaio: ≥95% (calc. to the dried substance)
• forma: powder
• Impurezas: ≤1% insoluble in ethanol
• perda: ≤22% loss on drying, 105 °C
• cor: very dark green
• solubilidade: ethanol: insoluble ≤1%
• aplicação(ões): diagnostic assay manufacturing; hematology; histology
• temperatura de armazenamento: room temp
• cadeia de caracteres SMILES: O.[Cl-].CN(C)c1ccc2N=C3C=C\C(C=C3Sc2c1)=[N+](/C)C
• InChI: 1S/C16H18N3S.ClH.H2O/c1-18(2)11-5-7-13-15(9-11)20-16-10-12(19(3)4)6-8-14(16)17-13;;/h5-10H,1-4H3;1H;1H2/q+1;;/p-1
• chave InChI: WQVSELLRAGBDLX-UHFFFAOYSA-M

Descrição

Descrição geral

Methylene blue is bis(dimethylamino)phenothiazine hydrochloride (tetramethylthionine),
C₁₆H₁₈N₃SCl. It is a major basic histological dye in pharmacology and microbiology. This heterocyclic aromatic chemical appears as a dark green solid powder, changing to blue in an aqueous solution. Methylene blue is tested for histological and and bacteriological staining, and as a constituent of Wright’s blood stain.

Aplicação

Methylene blue is widely used in several routine staining applications including Mallory’s phloxine-methylene blue on paraffin sections of tissue fixed in Zenker’s fluid for nuclear staining and bacterial staining in milk. It is also suitable for manufacturing the polychrome methylene blue used in the preparation of Romanowsky stains.Methylene Blue hydrate has also been used:

• in the preparation of methylene blue (MB) solution
• to stain zymograms -to label the injection site in the tissue slices
• to pre-treat mice via oral gavage to study its effects on noise-induced hearing loss

Informações de segurança

• Pictogramas: GHS07
• Palavra indicadora: Warning
• Frases de perigo: H302
• Declarações de precaução: P264 - P270 - P301 + P312 - P501
• Classificações de perigo: Acute Tox. 4 Oral
• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 3
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable
• Equipamento de proteção individual: dust mask type N95 (US), Eyeshields, Gloves