B5525

Bromophenol Blue sodium salt

• Sinônimo(s): 3′,3′′,5′,5′′-Tetrabromophenolsulfophthalein sodium salt
• Fórmula empírica (Notação de Hill): C₁₉H₉Br₄NaO₅S
• Número CAS: 34725-61-6
• Peso molecular: 691.94
• Beilstein: 6995701
• Número CE: 252-170-2
• Número MDL: MFCD00013793
• Código UNSPSC: 12171500
• ID de substância PubChem: 57653941
• NACRES: NA.52

Propriedades

• Formulário: powder
• Nível de qualidade: 200
• técnica(s): electrophoresis: suitable
• solubilidade: water: soluble
• adequação: suitable for nucleic acid electrophoresis
• temperatura de armazenamento: room temp
• cadeia de caracteres SMILES: [Na+].Oc1c(Br)cc(cc1Br)C(=C2/C=C(Br)C(=O)C(Br)=C2)\c3ccccc3S([O-])(=O)=O
• InChI: 1S/C19H10Br4O5S.Na/c20-12-5-9(6-13(21)18(12)24)17(10-7-14(22)19(25)15(23)8-10)11-3-1-2-4-16(11)29(26,27)28;/h1-8,24H,(H,26,27,28);/q;+1/p-1
• chave InChI: TWKDJMRHSLWRFI-UHFFFAOYSA-M

Descrição

Descrição geral

Bromophenol Blue is a pH indicator, and a dye appearing as a strong blue color. It is often used as a tracking dye during agarose or polyacrylamide gel electrophoresis. Bromophenol blue has a slight negative charge and will migrate the same direction as DNA, allowing the user to monitor the progress of molecules moving through the gel. The rate of migration varies with gel composition.

Aplicação

A tracking dye for nucleic acid or protein electrophoresis in agarose or polyacrylamide gels.

Bromophenol blue sodium salt has been used in the preparation of protein samples for western blotting analysis. It has also been used in Drosphila fly food to aid visualization of gut for dissection.

Reconstituição

A 6x Gel Loading Buffer can be made using standard recipes, including 0.015% bromophenol blue.

Informações de segurança

• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 3
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable
• Equipamento de proteção individual: Eyeshields, Gloves, type N95 (US)