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Documentos Principais

M0650

Sigma-Aldrich

Medium 199

With Earle′s salts, without ʟ-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture, 10X

Sinônimo(s):

Cell growth medium, M199 Medium, TCM 199

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About This Item

Código UNSPSC:
12352207
NACRES:
NA.75

Nome do produto

Medium 199, 10 ×, With Earle′s salts, without L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

Nível de qualidade

400

esterilidade

sterile-filtered

Formulário

liquid

concentração

10 ×

técnica(s)

cell culture | mammalian: suitable

Impurezas

endotoxin, tested

componentes

NaHCO3: no
phenol red: yes
sodium pyruvate: no
HEPES: no
Earle’s salts (5% CO2): yes
L-glutamine: no

Condições de expedição

ambient

temperatura de armazenamento

2-8°C

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Descrição geral

Medium 199 is a combination of vitamins, amino acids, and other factors that were originally developed as a completely defined media formulation for the culture of primary explants. This medium, when properly supplemented has broad applicability, particularly for non-transformed cells. Medium 199 is widely used for vaccine production, in vitro cultivation of primary pancreatic explants, and lens tissues.

Aplicação

Medium 199 has been used:

  • to culture human mesothelial cells
  • as a component of the holding medium for the collection of embryos from pluriparous Boer goat
  • in the preparation of spheroid-embedded collagen matrices

Reconstituição

Supplement with 0.1 g/L L-glutamine, 2.2 g/L sodium bicarbonate at 1×.

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suplemento

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Código de classe de armazenamento

12 - Non Combustible Liquids

Classe de risco de água (WGK)

WGK 2

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Escolha uma das versões mais recentes:

Certificados de análise (COA)

Lot/Batch Number
RNBL7705
RNBL1645
RNBK2084
RNBJ1355
RNBH5449

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