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Documentos Principais

L2380

Sigma-Aldrich

Lectin from Bandeiraea simplicifolia (Griffonia simplicifolia)

lyophilized powder

Sinônimo(s):

Bandeirea simplicifolia agglutinin, BS-I

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About This Item

Código UNSPSC:
12352202
NACRES:
NA.32

Formulário

lyophilized powder

Nível de qualidade

potência

<20 μg per mL agglutination activity (using human blood group B erythrocytes)

composição

Protein, ≥90% biuret

Impurezas

salt, free

temperatura de armazenamento

2-8°C

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Ações bioquímicas/fisiológicas

BS-I has a major affinity for terminal α-D-galactosyl residues with a secondary affinity for terminal N-acetyl-α-D-galactosaminyl residues.

Outras notas

BS-I is a tetrameric lectin consisting of two types of subunits designated A and B. There are five BS-I isolectins with different subunit composition: BSI-B4, BSI-AB3, BSI-A2B2, BSI-A3B and BSI-A4. BSI-B4 is blood group B specific and has an exclusive affinity for terminal α-D-galactosyl residues, whereas BSI-A4 has blood group A specificity and has a major affinity for terminal N-acetyl-α-D-galactosaminyl residues.

Nota de preparo

Purified by affinity chromatography using the method of Hayes, C. E., and Goldstein, I. J., J. Biol. Chem., 249, 1904 (1974).

Nota de análise

Agglutination activity is expressed in μg/mL and is determined from serial dilutions of a 1 mg/mL solution using phosphate buffered saline, pH 6.8, containing, for each lectin, calcium, magnesium, and manganese at different concentrations. This activity is the lowest concentration to agglutinate a 2% suspension of appropriate erythrocytes after 1 hr incubation at 25 °C.

Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves, type N95 (US)


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