L5391
Lectin from Bandeiraea simplicifolia (Griffonia simplicifolia)
Isolectin B4 (BSI-B4), peroxidase conjugate, lyophilized powder
Sinônimo(s):
Bandeirea simplicifolia agglutinin, BS-I
Faça loginpara ver os preços organizacionais e de contrato
About This Item
Código UNSPSC:
12352202
NACRES:
NA.32
Produtos recomendados
fonte biológica
Bandieraea simplicifolia
Nível de qualidade
conjugado
peroxidase conjugate
Ensaio
≥90% protein basis (Warburg-Christian)
Formulário
lyophilized powder
potência
<200 μg per mL agglutination activity (using human blood group B erythrocytes)
peroxidase activity
40-160 units/mg protein
composição
Protein, ~95% modified Warburg-Christian
técnica(s)
blood typing: suitable
fractionation: suitable
temperatura de armazenamento
−20°C
Procurando produtos similares? Visita Guia de comparação de produtos
Aplicação
General Western Blot Protocol:
- Glycoprotein sample size: 500ng
- Lectin Concentration: 0.1ug/ml
- Load samples at 500 ng of glycoprotein per lane
- Run 4-20% Bis-Tris SDS page gel
- Transfer gel to a PVDF membrane
- Block membrane for 1 hr at RT with RIPA buffer (R0278 Sigma)
- Incubate HRP lectin at 0.1ug/ml with RIPA buffer for 2 hours at RT
- Wash membrane 5 x 5 minutes with 25ml RIPA buffer
- Detect using chemiluminescent substrate (CPS1-120)
Ações bioquímicas/fisiológicas
BS-I has a major affinity for terminal α-D-galactosyl residues with a secondary affinity for terminal N-acetyl-α-D-galactosaminyl residues.
Outras notas
BS-I is a tetrameric lectin consisting of two types of subunits designated A and B. There are five BS-I isolectins with different subunit composition: BSI-B4, BSI-AB3, BSI-A2B2, BSI-A3B and BSI-A4. BSI-B4 is blood group B specific and has an exclusive affinity for terminal α-D-galactosyl residues, whereas BSI-A4 has blood group A specificity and has a major affinity for terminal N-acetyl-α-D-galactosaminyl residues.
Definição da unidade
One unit will form 1 mg purpurogallin in 20 sec from pyrogallol at pH 6.0 at 20 °C.
forma física
Contains sodium citrate buffer salts and calcium chloride
Nota de preparo
Prepared from peroxidase type VI using a modification of the method of O′Sullivan, et al. Repurified by affinity chromatography after conjugation.
Nota de análise
Agglutination activity is expressed in μg/mL and is determined from serial dilutions of a 1 mg/mL solution using phosphate buffered saline, pH 6.8, containing, for each lectin, calcium, magnesium, and manganese at different concentrations. This activity is the lowest concentration to agglutinate a 2% suspension of appropriate erythrocytes after 1 hr incubation at 25 °C.
Código de classe de armazenamento
11 - Combustible Solids
Classe de risco de água (WGK)
WGK 3
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
Equipamento de proteção individual
Eyeshields, Gloves, type N95 (US)
Escolha uma das versões mais recentes:
Já possui este produto?
Encontre a documentação dos produtos que você adquiriu recentemente na biblioteca de documentos.
Os clientes também visualizaram
Dariusz Orlowski et al.
Neuromodulation : journal of the International Neuromodulation Society, 20(5), 417-423 (2017-02-22)
The use of Deep Brain Stimulation (DBS) in treatment of various brain disorders is constantly growing; however, the number of studies of the reaction of the brain tissue toward implanted leads is still limited. Therefore, the aim of our study
Luís Fernándes de Castro et al.
Tissue engineering. Part A, 16(4), 1157-1168 (2009-10-29)
The parathyroid-hormone-related protein (PTHrP) is an important modulator of bone formation and bone remodeling. High and/or prolonged glucocorticoid (GC) treatments inhibit PTHrP expression in osteoblastic cells and bone formation and repair. We assessed the ability of the N- and C-terminal
Qing Wang et al.
Neurobiology of disease, 36(1), 223-231 (2009-07-29)
Pyruvate, a key intermediate in glucose metabolism, was explored as a potential treatment in models of experimental stroke and inflammation. Pyruvate was administered to rodents after the onset of middle cerebral artery occlusion (MCAO). Since the extent of inflammation is
Inflammation and NFkappaB activation is decreased by hypothermia following global cerebral ischemia.
Carla M Webster et al.
Neurobiology of disease, 33(2), 301-312 (2008-12-10)
We previously showed that hypothermia attenuates inflammation in focal cerebral ischemia (FCI) by suppressing activating kinases of nuclear factor-kappa B (NFkappaB). Here we characterize the inflammatory response in global cerebral ischemia (GCI), and the influence of mild hypothermia. Rodents were
Bruno Sevá Pessôa et al.
Journal of the American Heart Association, 4(2) (2015-02-07)
Angiotensin-(1-7) improves cardiac function and remodeling after myocardial infarction (MI). This may involve recruitment of hematopoietic progenitor cells that support angiogenesis. However, angiotensin-(1-7) is rapidly metabolized in plasma and tissue. The authors investigated in mice the effect of a metabolically
Nossa equipe de cientistas tem experiência em todas as áreas de pesquisa, incluindo Life Sciences, ciência de materiais, síntese química, cromatografia, química analítica e muitas outras.
Entre em contato com a assistência técnica