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Key Documents

K4002

Sigma-Aldrich

Krebs-Ringer Bicarbonate Buffer

With 1800 mg/L glucose, without calcium chloride and sodium bicarbonate, powder, suitable for cell culture

Sinônimo(s):

Bicarbonate Buffer Solution, Krebs Buffer, Krebs-Ringer Solution

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About This Item

Código UNSPSC:
12161702
NACRES:
NA.75

Nível de qualidade

forma

powder

técnica(s)

cell culture | mammalian: suitable

pH

6.1-6.7

solubilidade

water: 9.5 g/L, clear

temperatura de armazenamento

2-8°C

Descrição geral

Krebs-Ringer Bicarbonate Buffer acts as a salt solution and plays an essential role in tissue culture. A salt solution provides the cells with water and inorganic ions. It also helps in maintaining the osmotic balance and ideal pH in the medium. This bicarbonate buffered isotonic saline contains magnesium, potassium, sodium, and phosphate ions.

Aplicação

Krebs-Ringer Bicarbonate Buffer has been used:

  • as a wash buffer for the preparation of mice primary adipocytes and lipolysis assay
  • in the pre-perifusion for islet viability and functionality studies
  • in pituitary bioassay

Quantidade

Formulated to contain 9.5 grams of powder per liter of medium.

Reconstituição

Supplement with 1.26 g/L sodium bicarbonate.

Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 1

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable


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