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I2911

Sigma-Aldrich

StableCell™ IMDM

With stable glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

Sinônimo(s):

Iscove’s Modified Dulbecco’s Medium, IMDM

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About This Item

Número MDL:
MFCD00217469
Código UNSPSC:
12352207

Nível de qualidade

400

esterilidade

sterile-filtered

forma

liquid

técnica(s)

cell culture | hybridoma: suitable
cell culture | mammalian: suitable

Impurezas

Endotoxin, tested

componentes

NaHCO3: yes
glucose: yes
stable glutamine: yes
phenol red: yes

Condições de expedição

ambient

temperatura de armazenamento

2-8°C

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Descrição geral

This IMDM differs from the original formula, because it is supplemented with L-alanyl-L-glutamine dipeptide. Glutamine is notoriously unstable in cell culture. This dipeptide provides a more stable form of glutamine for use in cell cultures.

Aplicação

Guilbert and Iscove demonstrated that precursor cells of erythrocytes and macrophages could be cultured in a totally defined serum free medium supplemented with albumin, transferrin, lecithin, and selenium. This medium is a modification of Dulbecco′s Modified Eagle′s Medium (DME) containing selenium, additional amino acids and vitamins, sodium pyruvate, HEPES buffer, and potassium nitrate instead of ferric nitrate. Further studies demonstrated that Iscove′s Medium would support murine B lymphocytes, hemopoietic tissue from bone marrow, B cells stimulated with lipopolysaccharide, T lymphocytes, and a variety of hybrid cells.

Informações legais

StableCell is a trademark of Sigma-Aldrich Co. LLC

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Código de classe de armazenamento

12 - Non Combustible Liquids

Classe de risco de água (WGK)

WGK 1

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Certificados de análise (COA)

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