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Nutrient Mixture F-12 Ham

With ʟ-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

Sinônimo(s):

Ham’s F-12

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About This Item

Número MDL:
MFCD00217417
Código UNSPSC:
12352207
NACRES:
NA.75

product name

Nutrient Mixture F-12 Ham, With L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

esterilidade

sterile-filtered

forma

liquid

técnica(s)

cell culture | mammalian: suitable

Impurezas

endotoxin, tested

componentes

L-glutamine: yes
HEPES: no
phenol red: yes
sodium pyruvate: 0.11 g/L
NaHCO3: yes

Condições de expedição

ambient

temperatura de armazenamento

2-8°C

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Descrição geral

Ham′s Nutrient Mixtures were mainly created to support the clonal growth of numerous clones of Chinese hamster ovary (CHO) cells and clones of HeLa and mouse L-cells. It is also used to grow primary rat hepatocytes and rat prostate epithelial cells. Ham′s F-12 is used as the medium of choice for a clonal toxicity assay using CHO cells.

Aplicação

Nutrient Mixture F-12 Ham has been used:
  • to culture Chinese hamster ovary cells overexpressing insulin receptor (CHO-IR)
  • to culture LAN-1 cells
  • to maintain SUM149, SUM159, SUM190, FC-IBC02, BCX010, and IBC3 cells
  • with Dulbecco’s modified Eagle’s medium (DMEM) media in a 1:1 ratio to culture oral cavity squamous cell carcinoma (OSCC) cell lines.

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suplemento

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Código de classe de armazenamento

12 - Non Combustible Liquids

Classe de risco de água (WGK)

WGK 1

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

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WiNTRLINC1 is a long non-coding RNA (lncRNA) that positively regulates the Wnt pathway via achaete-scute complex homolog 2 (ASCL2) in colorectal cancer. ASCL2 was recently reported to play a critical role in chemoresistance, however clinical relevance of the WiNTRLINC1/ASCL2 axis
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Oncology letters, 14(1), 264-270 (2017-07-12)
Whether the poor prognosis of primary central nervous system lymphoma (PCNSL) compared with systemic diffuse large B cell lymphoma (DLBCL) is attributable to the immune privilege of the intracerebral location or to intrinsic differences in the biological characteristics of two
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Annals of surgical oncology, 26(5), 1401-1411 (2019-02-02)
Treatment-resistance genes limiting anticancer therapy have not been well clarified in colorectal cancer (CRC). We explored gene expression profiles to identify biomarkers for predicting treatment resistance to an anticancer drug in CRC. Six CRC cell lines were treated with phenylbutyrate
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Journal of lipid research, 59(5), 830-842 (2018-03-23)
apoE is the primary lipid carrier within the CNS and the strongest genetic risk factor for late onset Alzheimer's disease (AD). apoE is primarily lipidated via ABCA1, and both are under transcriptional regulation by the nuclear liver X receptor (LXR).
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